Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo gelfoam® assay

Fuminari Uehara, Yasunori Tome, Shinji Miwa, Yukihiko Hiroshima, Shuya Yano, Mako Yamamoto, Sumiyuki Mii, Hiroki Maehara, Michael Bouvet, Fuminori Kanaya, Robert M. Hoffman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We previously described a color-coded imaging model that can quantify the length of nascent blood vessels using Gelfoam® implanted in nestin-driven green fluorescent protein (ND-GFP) nude mice. In ND-GFP mice, nascent blood vessels are labeled with GFP. We report here that osteosarcoma cells promote angiogenesis in the Gelfoam® angiogenesis assay in ND-GFP mice. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic ND-GFP nude mice. Seven days after transplantation of Gelfoam®, skin flaps were made and human 143B osteosarcoma cells expressing green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in cytoplasm were injected into the transplanted Gelfoam®. The control-group mice had only implanted Gelfoam®. Skin flaps were made at days 14, 21, and 28 after transplantation of the Gelfoam® to allow imaging of vascularization in the Gelfoam® using a variable-magnification small animal imaging system and confocal fluorescence microscopy. ND-GFP expressing nascent blood vessels penetrated and spread into the Gelfoam® in a time-dependent manner in both control and osteosarcoma-implanted mice. ND-GFP expressing blood vessels in the Gelfoam® of the osteosarcoma-implanted mice were associated with the cancer cells and larger and longer than in the Gelfoam®-only implanted mice (P<0.01). The results presented in this report demonstrate strong angiogenesis induction by osteosarcoma cells and suggest this process is a potential therapeutic target for this disease. J. Cell. Biochem. 115: 1490-1494, 2014.

Original languageEnglish
Pages (from-to)1490-1494
Number of pages5
JournalJournal of Cellular Biochemistry
Volume115
Issue number9
DOIs
Publication statusPublished - Sep 2014
Externally publishedYes

Keywords

  • ANGIOGENESIS
  • CONFOCAL MICROSCOPY
  • GELFOAM®
  • GREEN FLUORESCENT PROTEIN
  • NESTIN
  • OSTEOSARCOMA
  • RED FLUORESCENT PROTEIN
  • TRANSGENIC NUDE MOUSE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Uehara, F., Tome, Y., Miwa, S., Hiroshima, Y., Yano, S., Yamamoto, M., Mii, S., Maehara, H., Bouvet, M., Kanaya, F., & Hoffman, R. M. (2014). Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo gelfoam® assay. Journal of Cellular Biochemistry, 115(9), 1490-1494. https://doi.org/10.1002/jcb.24799