Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo

Yoshinosuke Hamada, Kanako Yuki, Masayuki Okazaki, Wataru Fujitani, Takuya Matsumoto, Michiyo Kobashi Hashida, Kentaro Harutsugu, Kiyoshi Nokihara, Michiharu Daito, Nariaki Matsuura, Junzo Takahashi

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.

Original languageEnglish
Pages (from-to)650-655
Number of pages6
JournalDental Materials Journal
Volume23
Issue number4
Publication statusPublished - Dec 2004
Externally publishedYes

Fingerprint

SVVYGLR peptide
Osteopontin
Peptides
Endothelial cells
Blood vessels
Air Sacs
Amino acids
Assays
Skin
Adhesion
Alanine
Vascular Endothelial Growth Factor A
Blood Vessels
Tyrosine
Endothelial Cells
Air

Keywords

  • Angiogenesis
  • Endothelial cells
  • SVVYGLR

ASJC Scopus subject areas

  • Dentistry(all)
  • Biomaterials

Cite this

Hamada, Y., Yuki, K., Okazaki, M., Fujitani, W., Matsumoto, T., Kobashi Hashida, M., ... Takahashi, J. (2004). Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo. Dental Materials Journal, 23(4), 650-655.

Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo. / Hamada, Yoshinosuke; Yuki, Kanako; Okazaki, Masayuki; Fujitani, Wataru; Matsumoto, Takuya; Kobashi Hashida, Michiyo; Harutsugu, Kentaro; Nokihara, Kiyoshi; Daito, Michiharu; Matsuura, Nariaki; Takahashi, Junzo.

In: Dental Materials Journal, Vol. 23, No. 4, 12.2004, p. 650-655.

Research output: Contribution to journalArticle

Hamada, Y, Yuki, K, Okazaki, M, Fujitani, W, Matsumoto, T, Kobashi Hashida, M, Harutsugu, K, Nokihara, K, Daito, M, Matsuura, N & Takahashi, J 2004, 'Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo', Dental Materials Journal, vol. 23, no. 4, pp. 650-655.
Hamada Y, Yuki K, Okazaki M, Fujitani W, Matsumoto T, Kobashi Hashida M et al. Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo. Dental Materials Journal. 2004 Dec;23(4):650-655.
Hamada, Yoshinosuke ; Yuki, Kanako ; Okazaki, Masayuki ; Fujitani, Wataru ; Matsumoto, Takuya ; Kobashi Hashida, Michiyo ; Harutsugu, Kentaro ; Nokihara, Kiyoshi ; Daito, Michiharu ; Matsuura, Nariaki ; Takahashi, Junzo. / Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo. In: Dental Materials Journal. 2004 ; Vol. 23, No. 4. pp. 650-655.
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