Osteogenic protein-1 reduces intercellular adhesion molecule-1 messenger RNA expression, infarct size and TUNEL-positive cardiomyocytes in ischemia/reperfusion rat hearts

Junichi Hayashi, Shozo Kusachi, Takashi Murakami, Toru Miyoshi, Keigo Nakamura, Kazuya Koten, Hiroko Ogawa, Satoshi Hirohata, Yoshifumi Ninomiya, Yasushi Shiratori

Research output: Contribution to journalArticle

Abstract

Background: Osteogenic protein, a member of the transforming growth factor-beta superfamily, has been reported to decrease the expression of intercellular adhesive molecules and prevent neutrophil accumulation and activity in tissue injury. Objective: To examine the effects of osteogenic protein on ischemia/reperfusion in rat hearts. Methods: Reperfusion was established after a 90 min ligation of the proximal left coronary artery in rats. Recombinant human osteogenic protein-1 (200 μg/kg) was administered via the femoral vein just before reperfusion. Intercellular adhesion molecule-1 (ICAM-1) messenger RNA (mRNA) expression and infarct size were evaluated using Northern blotting and triphenyl tetrazolium chloride staining, respectively. Terminal deoxynucleotidyl transferase mediated biotin-16-2′-deoxyuridine-5′-triphosphate nick end labeling (TUNEL) staining was also performed. Results: In osteogenic protein-1 treated rats, the expression of ICAM-1 mRNA in ischemia/reperfusion hearts rapidly increased 4 h after reperfusion, although, the increase was lower than that observed in the vehicle-treated hearts (7.4±1.6-old versus 14.6±3.7-fold increase compared to the increase observed in preligation control hearts, respectively). Similarly, in day 1 and day 7 hearts, the increase in ICAM-1 mRNA expression was significantly lower in ischemia/reperfusion hearts from rats treated with osteogenic protein-1 than in vehicle-treated rats (2.5±0.1-fold versus 5.8±2.3-fold and 1.5±0.3-fold versus 3.5±0.2-fold, respectively). Infarct size in rats treated with osteogenic protein-1 was significantly smaller than that observed in rats treated with vehicle (13.1±1.2% versus 28.5±5.7% of the left ventricle, P

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalExperimental and Clinical Cardiology
Volume8
Issue number4
Publication statusPublished - 2003

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Bone Morphogenetic Protein 7
In Situ Nick-End Labeling
Intercellular Adhesion Molecule-1
Cardiac Myocytes
Reperfusion
Ischemia
Messenger RNA
Staining and Labeling
Femoral Vein
DNA Nucleotidylexotransferase
Transforming Growth Factor beta
Northern Blotting
Adhesives
Heart Ventricles
Ligation
Chlorides
Coronary Vessels
Proteins
Neutrophils
Wounds and Injuries

Keywords

  • Apoptosis
  • Inflammation
  • Ischemic heart disease
  • Matrix protein
  • Molecular biology

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Osteogenic protein-1 reduces intercellular adhesion molecule-1 messenger RNA expression, infarct size and TUNEL-positive cardiomyocytes in ischemia/reperfusion rat hearts. / Hayashi, Junichi; Kusachi, Shozo; Murakami, Takashi; Miyoshi, Toru; Nakamura, Keigo; Koten, Kazuya; Ogawa, Hiroko; Hirohata, Satoshi; Ninomiya, Yoshifumi; Shiratori, Yasushi.

In: Experimental and Clinical Cardiology, Vol. 8, No. 4, 2003, p. 195-200.

Research output: Contribution to journalArticle

Hayashi, Junichi ; Kusachi, Shozo ; Murakami, Takashi ; Miyoshi, Toru ; Nakamura, Keigo ; Koten, Kazuya ; Ogawa, Hiroko ; Hirohata, Satoshi ; Ninomiya, Yoshifumi ; Shiratori, Yasushi. / Osteogenic protein-1 reduces intercellular adhesion molecule-1 messenger RNA expression, infarct size and TUNEL-positive cardiomyocytes in ischemia/reperfusion rat hearts. In: Experimental and Clinical Cardiology. 2003 ; Vol. 8, No. 4. pp. 195-200.
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T1 - Osteogenic protein-1 reduces intercellular adhesion molecule-1 messenger RNA expression, infarct size and TUNEL-positive cardiomyocytes in ischemia/reperfusion rat hearts

AU - Hayashi, Junichi

AU - Kusachi, Shozo

AU - Murakami, Takashi

AU - Miyoshi, Toru

AU - Nakamura, Keigo

AU - Koten, Kazuya

AU - Ogawa, Hiroko

AU - Hirohata, Satoshi

AU - Ninomiya, Yoshifumi

AU - Shiratori, Yasushi

PY - 2003

Y1 - 2003

N2 - Background: Osteogenic protein, a member of the transforming growth factor-beta superfamily, has been reported to decrease the expression of intercellular adhesive molecules and prevent neutrophil accumulation and activity in tissue injury. Objective: To examine the effects of osteogenic protein on ischemia/reperfusion in rat hearts. Methods: Reperfusion was established after a 90 min ligation of the proximal left coronary artery in rats. Recombinant human osteogenic protein-1 (200 μg/kg) was administered via the femoral vein just before reperfusion. Intercellular adhesion molecule-1 (ICAM-1) messenger RNA (mRNA) expression and infarct size were evaluated using Northern blotting and triphenyl tetrazolium chloride staining, respectively. Terminal deoxynucleotidyl transferase mediated biotin-16-2′-deoxyuridine-5′-triphosphate nick end labeling (TUNEL) staining was also performed. Results: In osteogenic protein-1 treated rats, the expression of ICAM-1 mRNA in ischemia/reperfusion hearts rapidly increased 4 h after reperfusion, although, the increase was lower than that observed in the vehicle-treated hearts (7.4±1.6-old versus 14.6±3.7-fold increase compared to the increase observed in preligation control hearts, respectively). Similarly, in day 1 and day 7 hearts, the increase in ICAM-1 mRNA expression was significantly lower in ischemia/reperfusion hearts from rats treated with osteogenic protein-1 than in vehicle-treated rats (2.5±0.1-fold versus 5.8±2.3-fold and 1.5±0.3-fold versus 3.5±0.2-fold, respectively). Infarct size in rats treated with osteogenic protein-1 was significantly smaller than that observed in rats treated with vehicle (13.1±1.2% versus 28.5±5.7% of the left ventricle, P

AB - Background: Osteogenic protein, a member of the transforming growth factor-beta superfamily, has been reported to decrease the expression of intercellular adhesive molecules and prevent neutrophil accumulation and activity in tissue injury. Objective: To examine the effects of osteogenic protein on ischemia/reperfusion in rat hearts. Methods: Reperfusion was established after a 90 min ligation of the proximal left coronary artery in rats. Recombinant human osteogenic protein-1 (200 μg/kg) was administered via the femoral vein just before reperfusion. Intercellular adhesion molecule-1 (ICAM-1) messenger RNA (mRNA) expression and infarct size were evaluated using Northern blotting and triphenyl tetrazolium chloride staining, respectively. Terminal deoxynucleotidyl transferase mediated biotin-16-2′-deoxyuridine-5′-triphosphate nick end labeling (TUNEL) staining was also performed. Results: In osteogenic protein-1 treated rats, the expression of ICAM-1 mRNA in ischemia/reperfusion hearts rapidly increased 4 h after reperfusion, although, the increase was lower than that observed in the vehicle-treated hearts (7.4±1.6-old versus 14.6±3.7-fold increase compared to the increase observed in preligation control hearts, respectively). Similarly, in day 1 and day 7 hearts, the increase in ICAM-1 mRNA expression was significantly lower in ischemia/reperfusion hearts from rats treated with osteogenic protein-1 than in vehicle-treated rats (2.5±0.1-fold versus 5.8±2.3-fold and 1.5±0.3-fold versus 3.5±0.2-fold, respectively). Infarct size in rats treated with osteogenic protein-1 was significantly smaller than that observed in rats treated with vehicle (13.1±1.2% versus 28.5±5.7% of the left ventricle, P

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KW - Inflammation

KW - Ischemic heart disease

KW - Matrix protein

KW - Molecular biology

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