Osteogenic potential of cultured human periosteum-derived cells - A pilot study of human cell transplantation into a rat calvarial defect model

Yoshiro Sakata, Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Takashi Fujii, Eiki Yamachika, Toshio Sugahara

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Periosteum shows osteogenic potential and has received considerable attention as a grafting material for the repair of bone and joint defects. The osteogenic potential of cultured periosteal cells has also been reported. The findings of bone formation induced by cultured human periosteum-derived cells using a rat model are presented. Material and methods: Human mandibular periosteum was placed into a culture medium with 10% foetal bovine serum for 14 days. After reaching confluence, periosteal cells were re-suspended with 0.25% trypsin/EDTA and then re-cultured three dimensionally on a collagen sponge. The periosteal cell/collagen complex was grafted into rat calvarial defects and an immunosuppressant (FK506, 1.0 mg/kg/day) was administered intramuscularly. At 2, 3, and 5 weeks postoperatively, grafted tissue was extirpated and compared histologically and radiographically with tissue from a collagen-only grafted group. Results: In the experimental group, periosteal cells had proliferated and differentiated into osteogenic cells by 2 weeks post grafting. At 3 weeks, new bone formation was evident. By 5 weeks, bone growth was observed and new calcification was detected in the defect. Conclusion: Cultured human periosteum-derived cells showed osteogenic potential in a xenogeneic graft model using rat calvarial defects.

Original languageEnglish
Pages (from-to)461-465
Number of pages5
JournalJournal of Cranio-Maxillofacial Surgery
Volume34
Issue number8
DOIs
Publication statusPublished - Dec 2006

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Periosteum
Cell Transplantation
Collagen
Osteogenesis
Bone Development
Porifera
Tacrolimus
Immunosuppressive Agents
Edetic Acid
Trypsin
Culture Media
Cultured Cells
Joints
Transplants
Bone and Bones
Serum

Keywords

  • bone repair
  • critical sized defect
  • human cultured periosteum-derived cells

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology
  • Surgery

Cite this

Osteogenic potential of cultured human periosteum-derived cells - A pilot study of human cell transplantation into a rat calvarial defect model. / Sakata, Yoshiro; Ueno, Takaaki; Kagawa, Toshimasa; Kanou, Miwa; Fujii, Takashi; Yamachika, Eiki; Sugahara, Toshio.

In: Journal of Cranio-Maxillofacial Surgery, Vol. 34, No. 8, 12.2006, p. 461-465.

Research output: Contribution to journalArticle

Sakata, Yoshiro ; Ueno, Takaaki ; Kagawa, Toshimasa ; Kanou, Miwa ; Fujii, Takashi ; Yamachika, Eiki ; Sugahara, Toshio. / Osteogenic potential of cultured human periosteum-derived cells - A pilot study of human cell transplantation into a rat calvarial defect model. In: Journal of Cranio-Maxillofacial Surgery. 2006 ; Vol. 34, No. 8. pp. 461-465.
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