Osteoclast differentiation factor modulates cell cycle machinery and causes a delay in s phase progression in RAW264 cells.

E Meiyanto, M Hoshijima, T Ogawa, N Ishida, T Takeya

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Osteoclast differentiation factor (ODF) induces differentiation of mouse RAW264 cells to mature osteoclasts. To understand the mechanism controlling a coupling between withdrawal from the cell cycle and differentiation, we examined cell cycle progression and expression profiles of cell cycle regulatory genes at the initial phase in committed cells. ODF rapidly converted the hyperphosphorylated form of the retinoblastoma protein (pRb) into the hypophosphorylated form. The p21 protein was induced by ODF treatment in the same time course with that of dephosphorylation of pRb, followed by a sharp decline. After this period, a delayed entry of the S phase started accompanying the induction of CycD3 and cdk6 in differentiating cells. Hydroxyurea treatment indicated that the S phase entry was a prerequisite for osteoclast formation. Thus, ODF induces pleiotropic effects on cell cycle regulatory genes in RAW264 cells during the initial phase of the differentiation process to osteoclasts.
Original languageEnglish
Pages (from-to)278-83
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume282
Issue number1
DOIs
Publication statusPublished - Mar 23 2001

Fingerprint

RANK Ligand
Machinery
Cell Cycle
Osteoclasts
Cells
cdc Genes
Regulator Genes
S Phase
Genes
Retinoblastoma Protein
Hydroxyurea
Cell Differentiation
Proteins

Keywords

  • Animals
  • Carrier Proteins
  • Carrier Proteins: physiology
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Cycle Proteins: genetics
  • Cell Cycle: physiology
  • Cell Differentiation
  • Cell Division
  • Cell Division: drug effects
  • Cell Line
  • Gene Expression Profiling
  • Hydroxyurea
  • Hydroxyurea: pharmacology
  • Membrane Glycoproteins
  • Membrane Glycoproteins: physiology
  • Mice
  • Osteoclasts
  • Osteoclasts: cytology
  • Osteoclasts: drug effects
  • Phosphorylation
  • RANK Ligand
  • Receptor Activator of Nuclear Factor-kappa B
  • Retinoblastoma Protein
  • Retinoblastoma Protein: metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • S Phase

Cite this

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title = "Osteoclast differentiation factor modulates cell cycle machinery and causes a delay in s phase progression in RAW264 cells.",
abstract = "Osteoclast differentiation factor (ODF) induces differentiation of mouse RAW264 cells to mature osteoclasts. To understand the mechanism controlling a coupling between withdrawal from the cell cycle and differentiation, we examined cell cycle progression and expression profiles of cell cycle regulatory genes at the initial phase in committed cells. ODF rapidly converted the hyperphosphorylated form of the retinoblastoma protein (pRb) into the hypophosphorylated form. The p21 protein was induced by ODF treatment in the same time course with that of dephosphorylation of pRb, followed by a sharp decline. After this period, a delayed entry of the S phase started accompanying the induction of CycD3 and cdk6 in differentiating cells. Hydroxyurea treatment indicated that the S phase entry was a prerequisite for osteoclast formation. Thus, ODF induces pleiotropic effects on cell cycle regulatory genes in RAW264 cells during the initial phase of the differentiation process to osteoclasts.",
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T1 - Osteoclast differentiation factor modulates cell cycle machinery and causes a delay in s phase progression in RAW264 cells.

AU - Meiyanto, E

AU - Hoshijima, M

AU - Ogawa, T

AU - Ishida, N

AU - Takeya, T

PY - 2001/3/23

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N2 - Osteoclast differentiation factor (ODF) induces differentiation of mouse RAW264 cells to mature osteoclasts. To understand the mechanism controlling a coupling between withdrawal from the cell cycle and differentiation, we examined cell cycle progression and expression profiles of cell cycle regulatory genes at the initial phase in committed cells. ODF rapidly converted the hyperphosphorylated form of the retinoblastoma protein (pRb) into the hypophosphorylated form. The p21 protein was induced by ODF treatment in the same time course with that of dephosphorylation of pRb, followed by a sharp decline. After this period, a delayed entry of the S phase started accompanying the induction of CycD3 and cdk6 in differentiating cells. Hydroxyurea treatment indicated that the S phase entry was a prerequisite for osteoclast formation. Thus, ODF induces pleiotropic effects on cell cycle regulatory genes in RAW264 cells during the initial phase of the differentiation process to osteoclasts.

AB - Osteoclast differentiation factor (ODF) induces differentiation of mouse RAW264 cells to mature osteoclasts. To understand the mechanism controlling a coupling between withdrawal from the cell cycle and differentiation, we examined cell cycle progression and expression profiles of cell cycle regulatory genes at the initial phase in committed cells. ODF rapidly converted the hyperphosphorylated form of the retinoblastoma protein (pRb) into the hypophosphorylated form. The p21 protein was induced by ODF treatment in the same time course with that of dephosphorylation of pRb, followed by a sharp decline. After this period, a delayed entry of the S phase started accompanying the induction of CycD3 and cdk6 in differentiating cells. Hydroxyurea treatment indicated that the S phase entry was a prerequisite for osteoclast formation. Thus, ODF induces pleiotropic effects on cell cycle regulatory genes in RAW264 cells during the initial phase of the differentiation process to osteoclasts.

KW - Animals

KW - Carrier Proteins

KW - Carrier Proteins: physiology

KW - Cell Cycle

KW - Cell Cycle Proteins

KW - Cell Cycle Proteins: genetics

KW - Cell Cycle: physiology

KW - Cell Differentiation

KW - Cell Division

KW - Cell Division: drug effects

KW - Cell Line

KW - Gene Expression Profiling

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KW - Hydroxyurea: pharmacology

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KW - Mice

KW - Osteoclasts

KW - Osteoclasts: cytology

KW - Osteoclasts: drug effects

KW - Phosphorylation

KW - RANK Ligand

KW - Receptor Activator of Nuclear Factor-kappa B

KW - Retinoblastoma Protein

KW - Retinoblastoma Protein: metabolism

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - S Phase

U2 - 10.1006/bbrc.2001.4564

DO - 10.1006/bbrc.2001.4564

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