Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis

Akiko Kukita, Toshio Kukita, Mamoru Oouchida, Hidefumi Maeda, Hitomi Yatsuki, Osamu Kohashi

Research output: Contribution to journalArticle

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Abstract

The differentiation of osteoclasts is regulated by transcription factors expressed in cells of osteoclast lineage. We isolated here a potential transcription factor from a cDNA library of an enriched population of preosteoclasts and osteoclasts. The cDNA encodes a protein with N-terminal POZ domain and C-terminal Kruppel-like zinc fingers. We designate this protein as osteoclast-derived zinc finger (OCZF). OCZF was found to be rat homologue of mouse leukemia/lymphoma-related factor (LRF). Northern blot and in situ hybridization analysis showed OCZF mRNA at a high level in osteoclasts and kidney cells. OCZF had a nuclear targeting sequence and was localized in the nucleus of transfected cells. In addition, OCZF specifically bound to the guanine-rich consensus sequences of Egr-1 and c-Krox. Transient transfection assays indicate that OCZF can repress transcription activity like other POZ domain proteins. Furthermore, antisense but not sense phosphorothioate oligodeoxynucleotides (ODNs) for OCZF cDNA suppressed the formation of osteoclast-like multinucleated cells (MNCs) in bone marrow culture, whereas the same ODNs did not significantly affect the formation of macrophage polykaryons and mononuclear preosteoclast-like cells (POCs). These results suggest that OCZF is a unique transcription factor that plays an important role in the late stage of osteoclastogenesis.

Original languageEnglish
Pages (from-to)1987-1997
Number of pages11
JournalBlood
Volume94
Issue number6
Publication statusPublished - Sep 15 1999
Externally publishedYes

Fingerprint

Zinc Fingers
Osteoclasts
Osteogenesis
Zinc
Proteins
Transcription Factors
Oligodeoxyribonucleotides
BTB-POZ Domain
Complementary DNA
Macrophages
Consensus Sequence
Guanine
Cell Lineage
Transcription
Cell Nucleus
Gene Library
Cell culture
Bone Marrow Cells
Northern Blotting
In Situ Hybridization

ASJC Scopus subject areas

  • Hematology

Cite this

Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis. / Kukita, Akiko; Kukita, Toshio; Oouchida, Mamoru; Maeda, Hidefumi; Yatsuki, Hitomi; Kohashi, Osamu.

In: Blood, Vol. 94, No. 6, 15.09.1999, p. 1987-1997.

Research output: Contribution to journalArticle

Kukita, Akiko ; Kukita, Toshio ; Oouchida, Mamoru ; Maeda, Hidefumi ; Yatsuki, Hitomi ; Kohashi, Osamu. / Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis. In: Blood. 1999 ; Vol. 94, No. 6. pp. 1987-1997.
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