Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells

Takaaki Ogawa, Masaaki Tokuda, Kazuhito Tomizawa, Hideki Matsui, Toshifumi Itano, Ryoji Konishi, Shunichiro Nagahata, Osamu Hatase

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Abstract

The effects of three immunosuppressants (rapamycin, FK506 and cyclosporin A) on the proliferation and differentiation of rat osteoblasts-like osteosarcoma cell line, ROS 17/2.8 (ROS) cells were examined in vitro. All immunosuppressants showed a direct inhibition on the proliferation of ROS cells with different potencies. Growth inhibition by rapamycin was stronger than that by FK506 or cyclosporin A. Rapamycin caused a significant increase in alkaline phosphatase (ALP) activity and in the expression of osteopontin and osteocalcin mRNAs. FK506 caused a moderate increase in ALP activity and a decreased expression of osteopontin mRNA Cyclosporin A caused a decrease in ALP activity and in the expression of type 1 α 1 collagen mRNA. Our study indicates that rapamycin directly acts on ROS cells and induces osteoblastic differentiation, however, the effect of FK506 and cyclosporin A is weak. Rapamycin significantly enhances the differentiation induced by 1,25(OH)2-vitaminD3.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume249
Issue number1
DOIs
Publication statusPublished - Aug 10 1998
Externally publishedYes

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Osteoblasts
Osteosarcoma
Sirolimus
Rats
Tacrolimus
Cyclosporine
Alkaline Phosphatase
Osteopontin
Immunosuppressive Agents
Messenger RNA
Osteocalcin
Collagen
Cells
Cell Proliferation
Cell Line
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells. / Ogawa, Takaaki; Tokuda, Masaaki; Tomizawa, Kazuhito; Matsui, Hideki; Itano, Toshifumi; Konishi, Ryoji; Nagahata, Shunichiro; Hatase, Osamu.

In: Biochemical and Biophysical Research Communications, Vol. 249, No. 1, 10.08.1998, p. 226-230.

Research output: Contribution to journalArticle

Ogawa, T, Tokuda, M, Tomizawa, K, Matsui, H, Itano, T, Konishi, R, Nagahata, S & Hatase, O 1998, 'Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells', Biochemical and Biophysical Research Communications, vol. 249, no. 1, pp. 226-230. https://doi.org/10.1006/bbrc.1998.9118
Ogawa, Takaaki ; Tokuda, Masaaki ; Tomizawa, Kazuhito ; Matsui, Hideki ; Itano, Toshifumi ; Konishi, Ryoji ; Nagahata, Shunichiro ; Hatase, Osamu. / Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 249, No. 1. pp. 226-230.
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