Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells

Takaaki Ogawa, Masaaki Tokuda, Kazuhito Tomizawa, Hideki Matsui, Toshifumi Itano, Ryoji Konishi, Shunichiro Nagahata, Osamu Hatase

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82 Citations (Scopus)

Abstract

The effects of three immunosuppressants (rapamycin, FK506 and cyclosporin A) on the proliferation and differentiation of rat osteoblasts-like osteosarcoma cell line, ROS 17/2.8 (ROS) cells were examined in vitro. All immunosuppressants showed a direct inhibition on the proliferation of ROS cells with different potencies. Growth inhibition by rapamycin was stronger than that by FK506 or cyclosporin A. Rapamycin caused a significant increase in alkaline phosphatase (ALP) activity and in the expression of osteopontin and osteocalcin mRNAs. FK506 caused a moderate increase in ALP activity and a decreased expression of osteopontin mRNA Cyclosporin A caused a decrease in ALP activity and in the expression of type 1 α 1 collagen mRNA. Our study indicates that rapamycin directly acts on ROS cells and induces osteoblastic differentiation, however, the effect of FK506 and cyclosporin A is weak. Rapamycin significantly enhances the differentiation induced by 1,25(OH)2-vitaminD3.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume249
Issue number1
DOIs
Publication statusPublished - Aug 10 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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