OsFRDL1 expressed in nodes is required for distribution of iron to grains in rice

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Iron (Fe) is essential for plant growth and development, but the molecular mechanisms underlying its distribution to different organs are poorly understood. We found that OsFRDL1 (FERRIC REDUCTASE DEFECTIVE LIKE 1), a plasma membrane-localized transporter for citrate, was highly expressed in the upper nodes of rice at the reproductive growth stage. OsFRDL1 was expressed in most cells of enlarged vascular bundles, diffuse vascular bundles, and the interjacent parenchyma cell bridges of uppermost node I, as well as vascular tissues of the leaf blade, leaf sheath, peduncle, rachis, husk, and stamen. Knockout of OsFRDL1 decreased pollen viability and grain fertility when grown in a paddy field. Iron was deposited in the parenchyma cell bridges, a few of the cell layers of the parenchyma tissues outside of the bundle sheath of enlarged vascular bundles in node I in both the wild-type rice and osfrdl1 mutant, but the mutant accumulated more Fe than the wild-type rice in this area. A stem-fed experiment with stable isotope 57Fe showed that the distribution of Fe in the anther and panicle decreased in the knockout line, but that in the flag leaf it increased compared with the wild-type rice. Taken together, our results show that OsFRDL1 expressed in the upper nodes is required for the distribution of Fe in the panicles through solubilizing Fe deposited in the apoplastic part of nodes in rice.

Original languageEnglish
Pages (from-to)5485-5494
Number of pages10
JournalJournal of Experimental Botany
Volume67
Issue number18
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Iron
Blood Vessels
iron
vascular bundles
rice
parenchyma (plant tissue)
cells
mutants
Plant Development
Membrane Transport Proteins
vascular tissues
peduncle
stamens
Pollen
Growth and Development
solubilization
leaf blade
Citric Acid
Isotopes
citrates

Keywords

  • 57Fe distribution
  • Apoplastic Fe
  • citrate efflux
  • node
  • rice
  • transporter

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

OsFRDL1 expressed in nodes is required for distribution of iron to grains in rice. / Yokosho, Kengo; Yamaji, Naoki; Ma, Jian Feng.

In: Journal of Experimental Botany, Vol. 67, No. 18, 01.10.2016, p. 5485-5494.

Research output: Contribution to journalArticle

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