Oscillatory intracellular Ca2+ responses in living bone

Yoshihito Ishihara, Yasuyo Sugawara, Hiroshi Kamioka, Noriaki Kawanabe, Keiji Naruse, Takashi Yamashiro

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Intracellular calcium (Ca2+) is an important secondary messenger that modulates many cellular processes. Its oscillatory signaling is considered to participate in the regulation of many different cell functions, including bone metabolism. However, it is not entirely clear whether Ca2+ oscillations occur between osteoblasts and osteocytes in integrated bone tissues because of the complex mineralized matrices surrounding bone cells. To address this issue, we have recently developed a novel ex vivo realtime imaging system, which made it possible to observe repetitive and autonomous oscillations in the intracellular Ca2+ concentration ([Ca2+]i) in intact chick calvarial explants. This system revealed that Ca2+ release from intracellular stores plays a key role in Ca2+ oscillations in bone cells. Additionally, gap junctions are important for the maintenance of these oscillations in osteocytes but not in osteoblasts. In this review, we describe the dynamic oscillatory elevations of Ca2+ levels that occur in osteoblasts and osteocytes in living bone.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalJournal of Oral Biosciences
Volume56
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Osteocytes
Bone
Osteoblasts
Bone and Bones
Bone Matrix
Gap Junctions
Metabolism
Imaging systems
Maintenance
Calcium
Cells
Tissue

Keywords

  • Calcium oscillation
  • Gap junction
  • Intracellular calcium stores
  • Osteoblast
  • Osteocyte

ASJC Scopus subject areas

  • Dentistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine (miscellaneous)

Cite this

Oscillatory intracellular Ca2+ responses in living bone. / Ishihara, Yoshihito; Sugawara, Yasuyo; Kamioka, Hiroshi; Kawanabe, Noriaki; Naruse, Keiji; Yamashiro, Takashi.

In: Journal of Oral Biosciences, Vol. 56, No. 2, 2014, p. 49-53.

Research output: Contribution to journalArticle

Ishihara, Yoshihito ; Sugawara, Yasuyo ; Kamioka, Hiroshi ; Kawanabe, Noriaki ; Naruse, Keiji ; Yamashiro, Takashi. / Oscillatory intracellular Ca2+ responses in living bone. In: Journal of Oral Biosciences. 2014 ; Vol. 56, No. 2. pp. 49-53.
@article{742a2c37ef724eb4b72aad4d7cf9e3a9,
title = "Oscillatory intracellular Ca2+ responses in living bone",
abstract = "Intracellular calcium (Ca2+) is an important secondary messenger that modulates many cellular processes. Its oscillatory signaling is considered to participate in the regulation of many different cell functions, including bone metabolism. However, it is not entirely clear whether Ca2+ oscillations occur between osteoblasts and osteocytes in integrated bone tissues because of the complex mineralized matrices surrounding bone cells. To address this issue, we have recently developed a novel ex vivo realtime imaging system, which made it possible to observe repetitive and autonomous oscillations in the intracellular Ca2+ concentration ([Ca2+]i) in intact chick calvarial explants. This system revealed that Ca2+ release from intracellular stores plays a key role in Ca2+ oscillations in bone cells. Additionally, gap junctions are important for the maintenance of these oscillations in osteocytes but not in osteoblasts. In this review, we describe the dynamic oscillatory elevations of Ca2+ levels that occur in osteoblasts and osteocytes in living bone.",
keywords = "Calcium oscillation, Gap junction, Intracellular calcium stores, Osteoblast, Osteocyte",
author = "Yoshihito Ishihara and Yasuyo Sugawara and Hiroshi Kamioka and Noriaki Kawanabe and Keiji Naruse and Takashi Yamashiro",
year = "2014",
doi = "10.1016/j.job.2014.03.002",
language = "English",
volume = "56",
pages = "49--53",
journal = "Journal of Oral Biosciences",
issn = "1349-0079",
publisher = "Japanese Association for Oral Biology",
number = "2",

}

TY - JOUR

T1 - Oscillatory intracellular Ca2+ responses in living bone

AU - Ishihara, Yoshihito

AU - Sugawara, Yasuyo

AU - Kamioka, Hiroshi

AU - Kawanabe, Noriaki

AU - Naruse, Keiji

AU - Yamashiro, Takashi

PY - 2014

Y1 - 2014

N2 - Intracellular calcium (Ca2+) is an important secondary messenger that modulates many cellular processes. Its oscillatory signaling is considered to participate in the regulation of many different cell functions, including bone metabolism. However, it is not entirely clear whether Ca2+ oscillations occur between osteoblasts and osteocytes in integrated bone tissues because of the complex mineralized matrices surrounding bone cells. To address this issue, we have recently developed a novel ex vivo realtime imaging system, which made it possible to observe repetitive and autonomous oscillations in the intracellular Ca2+ concentration ([Ca2+]i) in intact chick calvarial explants. This system revealed that Ca2+ release from intracellular stores plays a key role in Ca2+ oscillations in bone cells. Additionally, gap junctions are important for the maintenance of these oscillations in osteocytes but not in osteoblasts. In this review, we describe the dynamic oscillatory elevations of Ca2+ levels that occur in osteoblasts and osteocytes in living bone.

AB - Intracellular calcium (Ca2+) is an important secondary messenger that modulates many cellular processes. Its oscillatory signaling is considered to participate in the regulation of many different cell functions, including bone metabolism. However, it is not entirely clear whether Ca2+ oscillations occur between osteoblasts and osteocytes in integrated bone tissues because of the complex mineralized matrices surrounding bone cells. To address this issue, we have recently developed a novel ex vivo realtime imaging system, which made it possible to observe repetitive and autonomous oscillations in the intracellular Ca2+ concentration ([Ca2+]i) in intact chick calvarial explants. This system revealed that Ca2+ release from intracellular stores plays a key role in Ca2+ oscillations in bone cells. Additionally, gap junctions are important for the maintenance of these oscillations in osteocytes but not in osteoblasts. In this review, we describe the dynamic oscillatory elevations of Ca2+ levels that occur in osteoblasts and osteocytes in living bone.

KW - Calcium oscillation

KW - Gap junction

KW - Intracellular calcium stores

KW - Osteoblast

KW - Osteocyte

UR - http://www.scopus.com/inward/record.url?scp=84901620371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901620371&partnerID=8YFLogxK

U2 - 10.1016/j.job.2014.03.002

DO - 10.1016/j.job.2014.03.002

M3 - Article

AN - SCOPUS:84901620371

VL - 56

SP - 49

EP - 53

JO - Journal of Oral Biosciences

JF - Journal of Oral Biosciences

SN - 1349-0079

IS - 2

ER -