Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice

Yosuke Toda, Yin Wang, Akira Takahashi, Yuya Kawai, Yasuomi Tada, Naoki Yamaji, Jian Feng Ma, Motoyuki Ashikari, Toshinori Kinoshita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H+-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H+-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H+-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H+-ATPase inhibitor vanadate. Using H+-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H+-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H+-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H+-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.

Original languageEnglish
Pages (from-to)1220-1230
Number of pages11
JournalPlant and Cell Physiology
Volume57
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

H-transporting ATP synthase
Proton-Translocating ATPases
guard cells
Oryza sativa
rice
blue light
Light
Threonine
Liliopsida
threonine
plasma membrane
Cell Membrane
Oryza
fusicoccin
Vanadates
Magnoliopsida
Atmosphere
gas exchange
phosphorylation
Protein Isoforms

Keywords

  • Dumbbell-type stomata
  • H-ATPase
  • Light-induced stomatal opening
  • Rice

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice. / Toda, Yosuke; Wang, Yin; Takahashi, Akira; Kawai, Yuya; Tada, Yasuomi; Yamaji, Naoki; Ma, Jian Feng; Ashikari, Motoyuki; Kinoshita, Toshinori.

In: Plant and Cell Physiology, Vol. 57, No. 6, 01.06.2016, p. 1220-1230.

Research output: Contribution to journalArticle

Toda, Y, Wang, Y, Takahashi, A, Kawai, Y, Tada, Y, Yamaji, N, Ma, JF, Ashikari, M & Kinoshita, T 2016, 'Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice', Plant and Cell Physiology, vol. 57, no. 6, pp. 1220-1230. https://doi.org/10.1093/pcp/pcw070
Toda, Yosuke ; Wang, Yin ; Takahashi, Akira ; Kawai, Yuya ; Tada, Yasuomi ; Yamaji, Naoki ; Ma, Jian Feng ; Ashikari, Motoyuki ; Kinoshita, Toshinori. / Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice. In: Plant and Cell Physiology. 2016 ; Vol. 57, No. 6. pp. 1220-1230.
@article{852a01029681444ba1ff208c70d5e1b5,
title = "Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice",
abstract = "The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H+-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H+-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H+-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H+-ATPase inhibitor vanadate. Using H+-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H+-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H+-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H+-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.",
keywords = "Dumbbell-type stomata, H-ATPase, Light-induced stomatal opening, Rice",
author = "Yosuke Toda and Yin Wang and Akira Takahashi and Yuya Kawai and Yasuomi Tada and Naoki Yamaji and Ma, {Jian Feng} and Motoyuki Ashikari and Toshinori Kinoshita",
year = "2016",
month = "6",
day = "1",
doi = "10.1093/pcp/pcw070",
language = "English",
volume = "57",
pages = "1220--1230",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice

AU - Toda, Yosuke

AU - Wang, Yin

AU - Takahashi, Akira

AU - Kawai, Yuya

AU - Tada, Yasuomi

AU - Yamaji, Naoki

AU - Ma, Jian Feng

AU - Ashikari, Motoyuki

AU - Kinoshita, Toshinori

PY - 2016/6/1

Y1 - 2016/6/1

N2 - The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H+-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H+-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H+-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H+-ATPase inhibitor vanadate. Using H+-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H+-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H+-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H+-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.

AB - The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H+-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H+-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H+-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H+-ATPase inhibitor vanadate. Using H+-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H+-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H+-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H+-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.

KW - Dumbbell-type stomata

KW - H-ATPase

KW - Light-induced stomatal opening

KW - Rice

UR - http://www.scopus.com/inward/record.url?scp=84992121766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992121766&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcw070

DO - 10.1093/pcp/pcw070

M3 - Article

VL - 57

SP - 1220

EP - 1230

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 6

ER -