Original: Origin of Osteoblasts Involved in the Mechanism of Ectopic Bone Formation Induced by KUSA/A1 Cells with Honeycomb Carrier

Andrea Rodriguez, Silvia Borkosky, Hitoshi Nagatsuka, Hidetsugu Tsujigiwa, Phuu Pwint Han, Mika Okauchi, Ryo Tamamura, Noriyuki Nagai, Hailong Hu, Keisuke Nakano, Toshiyuki Kawakami

Research output: Contribution to journalArticle

Abstract

The basic principle of bone tissue engineering is to use seeded stem cells in porous scaffold. Stem cells can proliferate and differentiate into various types of mature cells. A kind of stem cell called KUSA/A1 is a marrow stromal cell, capable of differentiating into three mesenchymal phenotypes: osteocyte, adipocyte, and myocyte by treating with 5-azacytidine in cell culture. Moreover, it has been reported that the mechanism of bone induction by KUSA/A1 cells is similar to intramembranous ossification. In order to clarify the origin of osteoblasts implicated in new bone formation, KUSA/A1 cells alone and combined with Honeycomb carrier were implanted in Transgenic Green Fluorescent Protein mice (GFP) mice. The presence of GFP positive host cells with osteoblastic morphology as well as GFP negative cells, clearly of KUSA/A1 cells in origin were observed around the bony trabeculae. These results indicated that the new bone was not only produced by KUSA/A1 cells but also by host cells from the surrounding connective tissues. To our knowledge, this is the first study to describe that host cells play an important role in ectopic bone induced by implanted marrow stromal cells, which would need special attention in bone tissue engineering.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalJournal of Hard Tissue Biology
Volume16
Issue number2
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Ectopic bone formation
  • Honeycomb carrier
  • Host cells
  • KUSA/A1 cell

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

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