Abstract
Odontogenesis and osteogenesis are two distinct yet closely related processes that share common signalling pathways including regulatory control by Cbfal. Although Cbfal is well-recognized as the master regulator of osteoblastic differentiation, little is known of its precise role during odontogenesis. We sought to clarify this by examining morphogenesis of teeth and matrix protein gene expression in cbfal-knock-out mice at ed 17.5 and day 0 of birth, and comparing it with presumptive mandible bone-forming site. Results demonstrated morphological and functional differentiation differences between these two processes: incisor tooth germ showed morphological and functional differentiation of odontoblasts with expression of osteopontin and osteocalcin whereas the presumptive mandible bone-forming site showed lack of osteoblastic differentiation, and no expression of these two matrix proteins. Stage-specific and cytodifferentiation differences were also observed between incisor and molar tooth germs: incisor tooth germ progressed to the bell stage with odontoblast and ameloblast differentiation, and expression of tooth-specific proteins, amelogenin and dentine sialophosphoprotein, whereas molar tooth germ showed maturational arrest at bud to cap stage. Present findings suggest that 1. The transcription factor Cbfa-1 is associated with morphogenesis of teeth and matrix protein gene expression. 2. Compared to the incisor tooth germ, the molar tooth germ is more strongly subjected to control by Cbfal, and 3. In Cbfal-knockout mice, the odontoblast-like cells in the incisor and the spindle cells in the presumptive mandible forming region showed different patterns of gene expression of matrix proteins common to teeth and bones.
| Original language | English |
|---|---|
| Pages (from-to) | 35-43 |
| Number of pages | 9 |
| Journal | Journal of Hard Tissue Biology |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2004 |
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Keywords
- cbfal-knockout mice
- Gene expression
- In situ hybridization
- Incisor
- Matrix proteins
- Molar
- tooth germ development
- Transmission electron microscope
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry
- Biomaterials
- Orthopedics and Sports Medicine
- Dentistry(all)
- Cell Biology
Cite this
Original : Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice. / Nagatsuka, Hitoshi; Kitamura, Yoshiko; Tsujigiwa, Hidetsugu; You-jin, Lee; Gunduz, Mehmet; Huang, B. Z.; Nagai, Noriyuki; Siar, Chong Huat; Komori, Toshifumi; Lefevre, Mathieu.
In: Journal of Hard Tissue Biology, Vol. 13, No. 1, 2004, p. 35-43.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Original
T2 - Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice
AU - Nagatsuka, Hitoshi
AU - Kitamura, Yoshiko
AU - Tsujigiwa, Hidetsugu
AU - You-jin, Lee
AU - Gunduz, Mehmet
AU - Huang, B. Z.
AU - Nagai, Noriyuki
AU - Siar, Chong Huat
AU - Komori, Toshifumi
AU - Lefevre, Mathieu
PY - 2004
Y1 - 2004
N2 - Odontogenesis and osteogenesis are two distinct yet closely related processes that share common signalling pathways including regulatory control by Cbfal. Although Cbfal is well-recognized as the master regulator of osteoblastic differentiation, little is known of its precise role during odontogenesis. We sought to clarify this by examining morphogenesis of teeth and matrix protein gene expression in cbfal-knock-out mice at ed 17.5 and day 0 of birth, and comparing it with presumptive mandible bone-forming site. Results demonstrated morphological and functional differentiation differences between these two processes: incisor tooth germ showed morphological and functional differentiation of odontoblasts with expression of osteopontin and osteocalcin whereas the presumptive mandible bone-forming site showed lack of osteoblastic differentiation, and no expression of these two matrix proteins. Stage-specific and cytodifferentiation differences were also observed between incisor and molar tooth germs: incisor tooth germ progressed to the bell stage with odontoblast and ameloblast differentiation, and expression of tooth-specific proteins, amelogenin and dentine sialophosphoprotein, whereas molar tooth germ showed maturational arrest at bud to cap stage. Present findings suggest that 1. The transcription factor Cbfa-1 is associated with morphogenesis of teeth and matrix protein gene expression. 2. Compared to the incisor tooth germ, the molar tooth germ is more strongly subjected to control by Cbfal, and 3. In Cbfal-knockout mice, the odontoblast-like cells in the incisor and the spindle cells in the presumptive mandible forming region showed different patterns of gene expression of matrix proteins common to teeth and bones.
AB - Odontogenesis and osteogenesis are two distinct yet closely related processes that share common signalling pathways including regulatory control by Cbfal. Although Cbfal is well-recognized as the master regulator of osteoblastic differentiation, little is known of its precise role during odontogenesis. We sought to clarify this by examining morphogenesis of teeth and matrix protein gene expression in cbfal-knock-out mice at ed 17.5 and day 0 of birth, and comparing it with presumptive mandible bone-forming site. Results demonstrated morphological and functional differentiation differences between these two processes: incisor tooth germ showed morphological and functional differentiation of odontoblasts with expression of osteopontin and osteocalcin whereas the presumptive mandible bone-forming site showed lack of osteoblastic differentiation, and no expression of these two matrix proteins. Stage-specific and cytodifferentiation differences were also observed between incisor and molar tooth germs: incisor tooth germ progressed to the bell stage with odontoblast and ameloblast differentiation, and expression of tooth-specific proteins, amelogenin and dentine sialophosphoprotein, whereas molar tooth germ showed maturational arrest at bud to cap stage. Present findings suggest that 1. The transcription factor Cbfa-1 is associated with morphogenesis of teeth and matrix protein gene expression. 2. Compared to the incisor tooth germ, the molar tooth germ is more strongly subjected to control by Cbfal, and 3. In Cbfal-knockout mice, the odontoblast-like cells in the incisor and the spindle cells in the presumptive mandible forming region showed different patterns of gene expression of matrix proteins common to teeth and bones.
KW - cbfal-knockout mice
KW - Gene expression
KW - In situ hybridization
KW - Incisor
KW - Matrix proteins
KW - Molar
KW - tooth germ development
KW - Transmission electron microscope
UR - http://www.scopus.com/inward/record.url?scp=58349099246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58349099246&partnerID=8YFLogxK
U2 - 10.2485/jhtb.13.35
DO - 10.2485/jhtb.13.35
M3 - Article
AN - SCOPUS:58349099246
VL - 13
SP - 35
EP - 43
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
SN - 1341-7649
IS - 1
ER -