Original: Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice

Hitoshi Nagatsuka, Yoshiko Kitamura, Hidetsugu Tsujigiwa, Lee You-jin, Mehmet Gunduz, B. Z. Huang, Noriyuki Nagai, Chong Huat Siar, Toshifumi Komori, Mathieu Lefevre

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Odontogenesis and osteogenesis are two distinct yet closely related processes that share common signalling pathways including regulatory control by Cbfal. Although Cbfal is well-recognized as the master regulator of osteoblastic differentiation, little is known of its precise role during odontogenesis. We sought to clarify this by examining morphogenesis of teeth and matrix protein gene expression in cbfal-knock-out mice at ed 17.5 and day 0 of birth, and comparing it with presumptive mandible bone-forming site. Results demonstrated morphological and functional differentiation differences between these two processes: incisor tooth germ showed morphological and functional differentiation of odontoblasts with expression of osteopontin and osteocalcin whereas the presumptive mandible bone-forming site showed lack of osteoblastic differentiation, and no expression of these two matrix proteins. Stage-specific and cytodifferentiation differences were also observed between incisor and molar tooth germs: incisor tooth germ progressed to the bell stage with odontoblast and ameloblast differentiation, and expression of tooth-specific proteins, amelogenin and dentine sialophosphoprotein, whereas molar tooth germ showed maturational arrest at bud to cap stage. Present findings suggest that 1. The transcription factor Cbfa-1 is associated with morphogenesis of teeth and matrix protein gene expression. 2. Compared to the incisor tooth germ, the molar tooth germ is more strongly subjected to control by Cbfal, and 3. In Cbfal-knockout mice, the odontoblast-like cells in the incisor and the spindle cells in the presumptive mandible forming region showed different patterns of gene expression of matrix proteins common to teeth and bones.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalJournal of Hard Tissue Biology
Volume13
Issue number1
DOIs
Publication statusPublished - 2004

Fingerprint

Tooth Germ
Gene expression
Knockout Mice
Incisor
Bone
Odontoblasts
Proteins
Gene Expression
Tooth
Odontogenesis
Mandible
Amelogenin
Osteopontin
Osteocalcin
Morphogenesis
Bone and Bones
Transcription Factors
Transcription factors
Ameloblasts
Osteogenesis

Keywords

  • cbfal-knockout mice
  • Gene expression
  • In situ hybridization
  • Incisor
  • Matrix proteins
  • Molar
  • tooth germ development
  • Transmission electron microscope

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

Cite this

Nagatsuka, H., Kitamura, Y., Tsujigiwa, H., You-jin, L., Gunduz, M., Huang, B. Z., ... Lefevre, M. (2004). Original: Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice. Journal of Hard Tissue Biology, 13(1), 35-43. https://doi.org/10.2485/jhtb.13.35

Original : Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice. / Nagatsuka, Hitoshi; Kitamura, Yoshiko; Tsujigiwa, Hidetsugu; You-jin, Lee; Gunduz, Mehmet; Huang, B. Z.; Nagai, Noriyuki; Siar, Chong Huat; Komori, Toshifumi; Lefevre, Mathieu.

In: Journal of Hard Tissue Biology, Vol. 13, No. 1, 2004, p. 35-43.

Research output: Contribution to journalArticle

Nagatsuka, H, Kitamura, Y, Tsujigiwa, H, You-jin, L, Gunduz, M, Huang, BZ, Nagai, N, Siar, CH, Komori, T & Lefevre, M 2004, 'Original: Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice', Journal of Hard Tissue Biology, vol. 13, no. 1, pp. 35-43. https://doi.org/10.2485/jhtb.13.35
Nagatsuka, Hitoshi ; Kitamura, Yoshiko ; Tsujigiwa, Hidetsugu ; You-jin, Lee ; Gunduz, Mehmet ; Huang, B. Z. ; Nagai, Noriyuki ; Siar, Chong Huat ; Komori, Toshifumi ; Lefevre, Mathieu. / Original : Gene Expression of Matrix Proteins in Cbfa1-Knockout Mice. In: Journal of Hard Tissue Biology. 2004 ; Vol. 13, No. 1. pp. 35-43.
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