Origin of wheat B-genome chromosomes inferred from RNA sequencing analysis of leaf transcripts from section Sitopsis species of Aegilops

Yuka Miki, Kentaro Yoshida, Nobuyuki Mizuno, Shuhei Nasuda, Kazuhiro Sato, Shigeo Takumi

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    Dramatic changes occasionally occur in intergenic regions leading to genomic alterations during speciation and will consequently obscure the ancestral species that have contributed to the formation of allopolyploid organisms. The S genome of five species of section Sitopsis of genus Aegilops is considered to be an origin of B-genome in cultivated tetraploid and hexaploid wheat species, although its actual donor is still unclear. Here, we attempted to elucidate phylogenetic relationship among Sitopsis species by performing RNA sequencing of the coding regions of each chromosome. Thus, genome-wide polymorphisms were extensively analyzed in 19 accessions of the Sitopsis species in reference to the tetraploid and hexaploid wheat B genome sequences and consequently were efficiently anchored to the B-genome chromosomes. The results of our genome-wide exon sequencing and resultant phylogenetic analysis indicate that Ae. speltoides is likely to be the direct donor of all chromosomes of the wheat B genome. Our results also indicate that the genome differentiation during wheat allopolyploidization from S to B proceeds at different speeds over the chromosomes rather than at constant rate and recombination could be a factor determining the speed. This observation is potentially generalized to genome differentiation during plant allopolyploid evolution.

    Original languageEnglish
    Pages (from-to)171-182
    Number of pages12
    JournalDNA Research
    Volume26
    Issue number2
    DOIs
    Publication statusPublished - Apr 1 2019

    Keywords

    • RNA sequencing
    • chromosomal synteny
    • genome differentiation
    • genome-wide polymorphisms
    • wheat

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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