Abstract
Low dark noise is a prerequisite for rod cells, which mediate our dim-light vision. The low dark noise is achieved by the extremely stable character of the rod visual pigment, rhodopsin, which evolved from less stable cone visual pigments. We have developed a biochemical method to quickly evaluate the thermal activation rate of visual pigments. Using an isomerization locked chromophore, we confirmed that thermal isomerization of the chromophore is the sole cause of thermal activation. Interestingly, we revealed an unexpected correlation between the thermal stability of the dark state and that of the active intermediate MetaII. Furthermore, we assessed key residues in rhodopsin and cone visual pigments by mutation analysis and identified two critical residues (E122 and I189) in the retinal binding pocket which account for the extremely low thermal activation rate of rhodopsin.
| Original language | English |
|---|---|
| Article number | 11081 |
| Journal | Scientific Reports |
| Volume | 5 |
| DOIs | |
| Publication status | Published - Jun 10 2015 |
| Externally published | Yes |
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Cite this
Origin of the low thermal isomerization rate of rhodopsin chromophore. / Yanagawa, Masataka; Kojima, Keiichi; Yamashita, Takahiro; Imamoto, Yasushi; Matsuyama, Take; Nakanishi, Koji; Yamano, Yumiko; Wada, Akimori; Sako, Yasushi; Shichida, Yoshinori.
In: Scientific Reports, Vol. 5, 11081, 10.06.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Origin of the low thermal isomerization rate of rhodopsin chromophore
AU - Yanagawa, Masataka
AU - Kojima, Keiichi
AU - Yamashita, Takahiro
AU - Imamoto, Yasushi
AU - Matsuyama, Take
AU - Nakanishi, Koji
AU - Yamano, Yumiko
AU - Wada, Akimori
AU - Sako, Yasushi
AU - Shichida, Yoshinori
PY - 2015/6/10
Y1 - 2015/6/10
N2 - Low dark noise is a prerequisite for rod cells, which mediate our dim-light vision. The low dark noise is achieved by the extremely stable character of the rod visual pigment, rhodopsin, which evolved from less stable cone visual pigments. We have developed a biochemical method to quickly evaluate the thermal activation rate of visual pigments. Using an isomerization locked chromophore, we confirmed that thermal isomerization of the chromophore is the sole cause of thermal activation. Interestingly, we revealed an unexpected correlation between the thermal stability of the dark state and that of the active intermediate MetaII. Furthermore, we assessed key residues in rhodopsin and cone visual pigments by mutation analysis and identified two critical residues (E122 and I189) in the retinal binding pocket which account for the extremely low thermal activation rate of rhodopsin.
AB - Low dark noise is a prerequisite for rod cells, which mediate our dim-light vision. The low dark noise is achieved by the extremely stable character of the rod visual pigment, rhodopsin, which evolved from less stable cone visual pigments. We have developed a biochemical method to quickly evaluate the thermal activation rate of visual pigments. Using an isomerization locked chromophore, we confirmed that thermal isomerization of the chromophore is the sole cause of thermal activation. Interestingly, we revealed an unexpected correlation between the thermal stability of the dark state and that of the active intermediate MetaII. Furthermore, we assessed key residues in rhodopsin and cone visual pigments by mutation analysis and identified two critical residues (E122 and I189) in the retinal binding pocket which account for the extremely low thermal activation rate of rhodopsin.
UR - http://www.scopus.com/inward/record.url?scp=84931275690&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84931275690&partnerID=8YFLogxK
U2 - 10.1038/srep11081
DO - 10.1038/srep11081
M3 - Article
C2 - 26061742
AN - SCOPUS:84931275690
VL - 5
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 11081
ER -