Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment

Takanori Eguchi, Chiharu Sogawa, Yuka Okusya, Kenta Uchibe, Ryosuke Iinuma, Kisho Ono, Keisuke Nakano, Jun Murakami, Manabu Itoh, Kazuya Arai, Toshifumi Fujiwara, Yuri Namba, Yoshiki Murata, Kazumi Ohyama, Manami Shimomura, Hirohiko Okamura, Masaharu Takigawa, Tetsuya Nakatsura, Ken ichi Kozaki, Kuniaki OkamotoStuart K. Calderwood

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Ability to form cellular aggregations such as tumorspheres and spheroids have been used as a morphological marker of malignant cancer cells and in particular cancer stem cells (CSC). However, the common definition of the types of cellular aggregation formed by cancer cells has not been available. We examined morphologies of 67 cell lines cultured on three dimensional morphology enhancing NanoCulture Plates (NCP) and classified the types of cellular aggregates that form. Among the 67 cell lines, 49 cell lines formed spheres or spheroids, 8 cell lines formed grape-like aggregation (GLA), 8 cell lines formed other types of aggregation, and 3 cell lines formed monolayer sheets. Seven GLA-forming cell lines were derived from adenocarcinoma among the 8 lines. A neuroendocrine adenocarcinoma cell line PC-3 formed asymmetric GLA with ductal structures on the NCPs and rapidly growing asymmetric tumors that metastasized to lymph nodes in immunocompromised mice. In contrast, another adenocarcinoma cell line DU-145 formed spheroids in vitro and spheroid-like tumors in vivo that did not metastasize to lymph nodes until day 50 after transplantation. Culture in the 3D nanoenvironment and in a defined stem cell medium enabled the neuroendocrine adenocarcinoma cells to form slowly growing large organoids that expressed multiple stem cell markers, neuroendocrine markers, intercellular adhesion molecules, and oncogenes in vitro. In contrast, the more commonly used 2D serum-contained environment reduced intercellular adhesion and induced mesenchymal transition and promoted rapid growth of the cells. In addition, the 3D stemness nanoenvironment promoted secretion of HSP90 and EpCAM-exosomes, a marker of CSC phenotype, from the neuroendocrine organoids. These findings indicate that the NCP-based 3D environment enables cells to form stem cell tumoroids with multipotency and model more accurately the in vivo tumor status at the levels of morphology and gene expression.

Original languageEnglish
Article numbere0191109
JournalPLoS One
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

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Organoids
exosomes
Exosomes
Neoplastic Stem Cells
Stem cells
stem cells
Cells
cell lines
Cell Line
adenocarcinoma
Agglomeration
Vitis
Adenocarcinoma
grapes
Neuroendocrine Cells
Stem Cells
Neoplasms
Tumors
neoplasms
lymph nodes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment. / Eguchi, Takanori; Sogawa, Chiharu; Okusya, Yuka; Uchibe, Kenta; Iinuma, Ryosuke; Ono, Kisho; Nakano, Keisuke; Murakami, Jun; Itoh, Manabu; Arai, Kazuya; Fujiwara, Toshifumi; Namba, Yuri; Murata, Yoshiki; Ohyama, Kazumi; Shimomura, Manami; Okamura, Hirohiko; Takigawa, Masaharu; Nakatsura, Tetsuya; Kozaki, Ken ichi; Okamoto, Kuniaki; Calderwood, Stuart K.

In: PLoS One, Vol. 13, No. 2, e0191109, 01.02.2018.

Research output: Contribution to journalArticle

Eguchi, T, Sogawa, C, Okusya, Y, Uchibe, K, Iinuma, R, Ono, K, Nakano, K, Murakami, J, Itoh, M, Arai, K, Fujiwara, T, Namba, Y, Murata, Y, Ohyama, K, Shimomura, M, Okamura, H, Takigawa, M, Nakatsura, T, Kozaki, KI, Okamoto, K & Calderwood, SK 2018, 'Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment', PLoS One, vol. 13, no. 2, e0191109. https://doi.org/10.1371/journal.pone.0191109
Eguchi, Takanori ; Sogawa, Chiharu ; Okusya, Yuka ; Uchibe, Kenta ; Iinuma, Ryosuke ; Ono, Kisho ; Nakano, Keisuke ; Murakami, Jun ; Itoh, Manabu ; Arai, Kazuya ; Fujiwara, Toshifumi ; Namba, Yuri ; Murata, Yoshiki ; Ohyama, Kazumi ; Shimomura, Manami ; Okamura, Hirohiko ; Takigawa, Masaharu ; Nakatsura, Tetsuya ; Kozaki, Ken ichi ; Okamoto, Kuniaki ; Calderwood, Stuart K. / Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment. In: PLoS One. 2018 ; Vol. 13, No. 2.
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AU - Iinuma, Ryosuke

AU - Ono, Kisho

AU - Nakano, Keisuke

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AU - Fujiwara, Toshifumi

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AU - Calderwood, Stuart K.

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