Organelle DNA degradation contributes to the efficient use of phosphate in seed plants

Tsuneaki Takami, Norikazu Ohnishi, Yuko Kurita, Shoko Iwamura, Miwa Ohnishi, Makoto Kusaba, Tetsuro Mimura, Wataru Sakamoto

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    Mitochondria and chloroplasts (plastids) both harbour extranuclear DNA that originates from the ancestral endosymbiotic bacteria. These organelle DNAs (orgDNAs) encode limited genetic information but are highly abundant, with multiple copies in vegetative tissues, such as mature leaves. Abundant orgDNA constitutes a substantial pool of organic phosphate along with RNA in chloroplasts, which could potentially contribute to phosphate recycling when it is degraded and relocated. However, whether orgDNA is degraded nucleolytically in leaves remains unclear. In this study, we revealed the prevailing mechanism in which organelle exonuclease DPD1 degrades abundant orgDNA during leaf senescence. The DPD1 degradation system is conserved in seed plants and, more remarkably, we found that it was correlated with the efficient use of phosphate when plants were exposed to nutrient-deficient conditions. The loss of DPD1 compromised both the relocation of phosphorus to upper tissues and the response to phosphate starvation, resulting in reduced plant fitness. Our findings highlighted that DNA is also an internal phosphate-rich reservoir retained in organelles since their endosymbiotic origin.

    Original languageEnglish
    Pages (from-to)1044-1055
    Number of pages12
    JournalNature Plants
    Volume4
    Issue number12
    DOIs
    Publication statusPublished - Dec 1 2018

    ASJC Scopus subject areas

    • Plant Science

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