Organelle DNA degradation contributes to the efficient use of phosphate in seed plants

Tsuneaki Takami, Norikazu Ohnishi, Yuko Kurita, Shoko Iwamura, Miwa Ohnishi, Makoto Kusaba, Tetsuro Mimura, Wataru Sakamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mitochondria and chloroplasts (plastids) both harbour extranuclear DNA that originates from the ancestral endosymbiotic bacteria. These organelle DNAs (orgDNAs) encode limited genetic information but are highly abundant, with multiple copies in vegetative tissues, such as mature leaves. Abundant orgDNA constitutes a substantial pool of organic phosphate along with RNA in chloroplasts, which could potentially contribute to phosphate recycling when it is degraded and relocated. However, whether orgDNA is degraded nucleolytically in leaves remains unclear. In this study, we revealed the prevailing mechanism in which organelle exonuclease DPD1 degrades abundant orgDNA during leaf senescence. The DPD1 degradation system is conserved in seed plants and, more remarkably, we found that it was correlated with the efficient use of phosphate when plants were exposed to nutrient-deficient conditions. The loss of DPD1 compromised both the relocation of phosphorus to upper tissues and the response to phosphate starvation, resulting in reduced plant fitness. Our findings highlighted that DNA is also an internal phosphate-rich reservoir retained in organelles since their endosymbiotic origin.

Original languageEnglish
JournalNature Plants
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Spermatophytina
organelles
phosphates
degradation
DNA
chloroplasts
leaves
recycling
plastids
starvation
mitochondria
RNA
phosphorus
bacteria
nutrients
tissues

ASJC Scopus subject areas

  • Plant Science

Cite this

Organelle DNA degradation contributes to the efficient use of phosphate in seed plants. / Takami, Tsuneaki; Ohnishi, Norikazu; Kurita, Yuko; Iwamura, Shoko; Ohnishi, Miwa; Kusaba, Makoto; Mimura, Tetsuro; Sakamoto, Wataru.

In: Nature Plants, 01.01.2018.

Research output: Contribution to journalArticle

Takami, Tsuneaki ; Ohnishi, Norikazu ; Kurita, Yuko ; Iwamura, Shoko ; Ohnishi, Miwa ; Kusaba, Makoto ; Mimura, Tetsuro ; Sakamoto, Wataru. / Organelle DNA degradation contributes to the efficient use of phosphate in seed plants. In: Nature Plants. 2018.
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