Orchid fleck virus is a rhabdovirus with an unusual bipartite genome

Hideki Kondo, Takanori Maeda, Yukio Shirako, Tetsuo Tamada

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Orchid fleck virus (OFV) has an unusual bipartite negative-sense RNA genome with clear sequence similarities to those nucleorhabdoviruses. The OFV genome consists of two single-stranded RNA molecules, RNA1 and RNA2 that are 6413 and 6001 nt long, respectively, with open reading frame (ORF) information in the complementary sense. RNA1 encodes 49 (ORF1), 26 (ORF2), 38 (ORF3), 20 (ORF4) and 61 kDa (ORF5) proteins, and RNA2 encodes a single protein of 212 kDa (ORF6). ORF1, ORF5 and ORF6 proteins had significant similarities (21-38% identity) to the nucleocapsid protein (N), glycoprotein (G) and polymerase L gene products, respectively, of other rhabdoviruses, especially nucleorhabdoviruses, whereas ORF2, ORF3 and ORF4 proteins had no significant similarities to other proteins in the international databases. Similarities between OFV and rhabdoviruses were also found in the sequence complementarity at both termini of each RNA segment (the common terminal sequences are 3′-UGUGUC-GACACA-5′), the conserved intergenic sequences and in being negative sense. It was proposed that a new genus Dichorhabdovirus in the family Rhabdoviridae of the order Mononegavirales should be established with OFV as its prototype member and type species.

Original languageEnglish
Pages (from-to)2413-2421
Number of pages9
JournalJournal of General Virology
Volume87
Issue number8
DOIs
Publication statusPublished - Aug 2006

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Rhabdoviridae
Genome
Viruses
RNA
Proteins
Mononegavirales
Nucleocapsid Proteins
Intergenic DNA
Conserved Sequence
Open Reading Frames
Glycoproteins
Flecks
Databases
Genes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Orchid fleck virus is a rhabdovirus with an unusual bipartite genome. / Kondo, Hideki; Maeda, Takanori; Shirako, Yukio; Tamada, Tetsuo.

In: Journal of General Virology, Vol. 87, No. 8, 08.2006, p. 2413-2421.

Research output: Contribution to journalArticle

Kondo, Hideki ; Maeda, Takanori ; Shirako, Yukio ; Tamada, Tetsuo. / Orchid fleck virus is a rhabdovirus with an unusual bipartite genome. In: Journal of General Virology. 2006 ; Vol. 87, No. 8. pp. 2413-2421.
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