Orchid fleck virus is a rhabdovirus with an unusual bipartite genome

Hideki Kondo, Takanori Maeda, Yukio Shirako, Tetsuo Tamada

    Research output: Contribution to journalArticlepeer-review

    76 Citations (Scopus)

    Abstract

    Orchid fleck virus (OFV) has an unusual bipartite negative-sense RNA genome with clear sequence similarities to those nucleorhabdoviruses. The OFV genome consists of two single-stranded RNA molecules, RNA1 and RNA2 that are 6413 and 6001 nt long, respectively, with open reading frame (ORF) information in the complementary sense. RNA1 encodes 49 (ORF1), 26 (ORF2), 38 (ORF3), 20 (ORF4) and 61 kDa (ORF5) proteins, and RNA2 encodes a single protein of 212 kDa (ORF6). ORF1, ORF5 and ORF6 proteins had significant similarities (21-38% identity) to the nucleocapsid protein (N), glycoprotein (G) and polymerase L gene products, respectively, of other rhabdoviruses, especially nucleorhabdoviruses, whereas ORF2, ORF3 and ORF4 proteins had no significant similarities to other proteins in the international databases. Similarities between OFV and rhabdoviruses were also found in the sequence complementarity at both termini of each RNA segment (the common terminal sequences are 3′-UGUGUC-GACACA-5′), the conserved intergenic sequences and in being negative sense. It was proposed that a new genus Dichorhabdovirus in the family Rhabdoviridae of the order Mononegavirales should be established with OFV as its prototype member and type species.

    Original languageEnglish
    Pages (from-to)2413-2421
    Number of pages9
    JournalJournal of General Virology
    Volume87
    Issue number8
    DOIs
    Publication statusPublished - Aug 2006

    ASJC Scopus subject areas

    • Virology

    Fingerprint

    Dive into the research topics of 'Orchid fleck virus is a rhabdovirus with an unusual bipartite genome'. Together they form a unique fingerprint.

    Cite this