Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog

A case report

Koji Sugita, Nanako Yanuma, Hikaru Ohno, Kaho Takahashi, Koji Kawano, Hidetoshi Morita, Keitaro Ohmori

Research output: Contribution to journalArticle

Abstract

Background: Successful clinical outcomes of faecal microbiota transplantation (FMT) for recurrent Clostridium difficile infection have been reported in humans and a marmoset. However, it has been unclear whether oral FMT was effective for the treatment of C. difficile-associated diarrhoea in dogs. Case presentation: An 8-month-old, intact male French bulldog was presented with a 4-month history of intermittent large bowel diarrhoea. Physical and clinical examinations did not identify any specific causes for diarrhoea. Real-time PCR analysis and immunochromatography detected C. difficile antigen and toxin A&B genes and proteins in a faecal sample. Based on these findings, diarrhoea in the dog was considered to be induced by C. difficile-associated colitis. The dog was treated with oral FMT, in which a faecal solution obtained from a healthy beagle was orally administered to the subject. Stool consistency and frequency and faecal blood and mucus became normal 2-3 days after oral FMT, and real-time PCR analysis and immunochromatography was negative for C. difficile antigen and toxin A&B genes and proteins. No adverse events were observed. Conclusion: The present case report demonstrated that oral FMT was an effective treatment for C. difficile-associated diarrhoea in a dog. The findings in this report provide a rationale to evaluate clinical efficacy of oral FMT for other gastrointestinal diseases in dogs.

Original languageEnglish
Article number11
JournalBMC Veterinary Research
Volume15
Issue number1
DOIs
Publication statusPublished - Jan 7 2019

Fingerprint

Clostridium difficile
Diarrhea
diarrhea
mouth
Dogs
case studies
dogs
Immunochromatography
immunoaffinity chromatography
clinical examination
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
toxins
Therapeutics
antigens
Clostridium Infections
Antigens
Callithrix
Gastrointestinal Diseases
Callitrichidae

Keywords

  • Clostridium difficile
  • Diarrhoea
  • Dog
  • Oral faecal microbiota transplantation

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog : A case report. / Sugita, Koji; Yanuma, Nanako; Ohno, Hikaru; Takahashi, Kaho; Kawano, Koji; Morita, Hidetoshi; Ohmori, Keitaro.

In: BMC Veterinary Research, Vol. 15, No. 1, 11, 07.01.2019.

Research output: Contribution to journalArticle

Sugita, Koji ; Yanuma, Nanako ; Ohno, Hikaru ; Takahashi, Kaho ; Kawano, Koji ; Morita, Hidetoshi ; Ohmori, Keitaro. / Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog : A case report. In: BMC Veterinary Research. 2019 ; Vol. 15, No. 1.
@article{5bb6843c08f3445096a3e517984f45a6,
title = "Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog: A case report",
abstract = "Background: Successful clinical outcomes of faecal microbiota transplantation (FMT) for recurrent Clostridium difficile infection have been reported in humans and a marmoset. However, it has been unclear whether oral FMT was effective for the treatment of C. difficile-associated diarrhoea in dogs. Case presentation: An 8-month-old, intact male French bulldog was presented with a 4-month history of intermittent large bowel diarrhoea. Physical and clinical examinations did not identify any specific causes for diarrhoea. Real-time PCR analysis and immunochromatography detected C. difficile antigen and toxin A&B genes and proteins in a faecal sample. Based on these findings, diarrhoea in the dog was considered to be induced by C. difficile-associated colitis. The dog was treated with oral FMT, in which a faecal solution obtained from a healthy beagle was orally administered to the subject. Stool consistency and frequency and faecal blood and mucus became normal 2-3 days after oral FMT, and real-time PCR analysis and immunochromatography was negative for C. difficile antigen and toxin A&B genes and proteins. No adverse events were observed. Conclusion: The present case report demonstrated that oral FMT was an effective treatment for C. difficile-associated diarrhoea in a dog. The findings in this report provide a rationale to evaluate clinical efficacy of oral FMT for other gastrointestinal diseases in dogs.",
keywords = "Clostridium difficile, Diarrhoea, Dog, Oral faecal microbiota transplantation",
author = "Koji Sugita and Nanako Yanuma and Hikaru Ohno and Kaho Takahashi and Koji Kawano and Hidetoshi Morita and Keitaro Ohmori",
year = "2019",
month = "1",
day = "7",
doi = "10.1186/s12917-018-1754-z",
language = "English",
volume = "15",
journal = "BMC Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog

T2 - A case report

AU - Sugita, Koji

AU - Yanuma, Nanako

AU - Ohno, Hikaru

AU - Takahashi, Kaho

AU - Kawano, Koji

AU - Morita, Hidetoshi

AU - Ohmori, Keitaro

PY - 2019/1/7

Y1 - 2019/1/7

N2 - Background: Successful clinical outcomes of faecal microbiota transplantation (FMT) for recurrent Clostridium difficile infection have been reported in humans and a marmoset. However, it has been unclear whether oral FMT was effective for the treatment of C. difficile-associated diarrhoea in dogs. Case presentation: An 8-month-old, intact male French bulldog was presented with a 4-month history of intermittent large bowel diarrhoea. Physical and clinical examinations did not identify any specific causes for diarrhoea. Real-time PCR analysis and immunochromatography detected C. difficile antigen and toxin A&B genes and proteins in a faecal sample. Based on these findings, diarrhoea in the dog was considered to be induced by C. difficile-associated colitis. The dog was treated with oral FMT, in which a faecal solution obtained from a healthy beagle was orally administered to the subject. Stool consistency and frequency and faecal blood and mucus became normal 2-3 days after oral FMT, and real-time PCR analysis and immunochromatography was negative for C. difficile antigen and toxin A&B genes and proteins. No adverse events were observed. Conclusion: The present case report demonstrated that oral FMT was an effective treatment for C. difficile-associated diarrhoea in a dog. The findings in this report provide a rationale to evaluate clinical efficacy of oral FMT for other gastrointestinal diseases in dogs.

AB - Background: Successful clinical outcomes of faecal microbiota transplantation (FMT) for recurrent Clostridium difficile infection have been reported in humans and a marmoset. However, it has been unclear whether oral FMT was effective for the treatment of C. difficile-associated diarrhoea in dogs. Case presentation: An 8-month-old, intact male French bulldog was presented with a 4-month history of intermittent large bowel diarrhoea. Physical and clinical examinations did not identify any specific causes for diarrhoea. Real-time PCR analysis and immunochromatography detected C. difficile antigen and toxin A&B genes and proteins in a faecal sample. Based on these findings, diarrhoea in the dog was considered to be induced by C. difficile-associated colitis. The dog was treated with oral FMT, in which a faecal solution obtained from a healthy beagle was orally administered to the subject. Stool consistency and frequency and faecal blood and mucus became normal 2-3 days after oral FMT, and real-time PCR analysis and immunochromatography was negative for C. difficile antigen and toxin A&B genes and proteins. No adverse events were observed. Conclusion: The present case report demonstrated that oral FMT was an effective treatment for C. difficile-associated diarrhoea in a dog. The findings in this report provide a rationale to evaluate clinical efficacy of oral FMT for other gastrointestinal diseases in dogs.

KW - Clostridium difficile

KW - Diarrhoea

KW - Dog

KW - Oral faecal microbiota transplantation

UR - http://www.scopus.com/inward/record.url?scp=85059759175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059759175&partnerID=8YFLogxK

U2 - 10.1186/s12917-018-1754-z

DO - 10.1186/s12917-018-1754-z

M3 - Article

VL - 15

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

IS - 1

M1 - 11

ER -