Oral administration of the probiotic bacterium Lactobacillus acidophilus strain L-55 modulates the immunological parameters of the laying hen inoculated with a Newcastle disease virus-based live attenuated vaccine

Dung Thi Ho, Toshimitsu Hatabu, Yosuke Sunada, Yasuhiro Kondo

Research output: Contribution to journalArticlepeer-review

Abstract

Probiotic supplements containing living bacteria have attracted interest as a potential source of health benefits for humans and livestock. The aim of this study was to determine whether administration of Lactobacillus acidophilus strain L-55 (LaL-55) enhances the immune response among chicks exposed to a Newcastle disease virus (NDV)- based live attenuated vaccine. Oral administration of LaL-55 augmented the elevation in the total numbers of leukocytes and lymphocytes following inoculation with the NDV-based live attenuated vaccine. Monocyte counts increased after LaL-55 administration independent of inoculation with the NDV vaccine. Among chicks that were administered LaL-55, there was a dose-dependent increase in the NK cell activity measured by a 51Cr release assay at 2 weeks after the secondary NDV vaccine inoculation. Two weeks after the secondary inoculation with the NDV vaccine, interferon (IFN)-γ-mRNA expression was significantly elevated in mononuclear splenocytes from chicks that were administered LaL-55. Meanwhile, LaL-55 administration did not change the mRNA levels of IFN-α, IFN-β, and interleukin-1β. These results may suggest that coadministration of LaL-55 with an NDV vaccine augments the immune response against the virus. Therefore, LaL-55 may help protect against viral diseases in poultry.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalBioscience of Microbiota, Food and Health
Volume39
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • Chicken
  • Immunomodulatory effect
  • Lactobacillus acidophilus L-55
  • Newcastle disease vaccine

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Gastroenterology

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