Oral administration of phenolic antidiarrheic ingredients prevents ovariectomy-induced bone loss

Nobuaki Moriguchi, Eiichi Hinoi, Takeshi Takarada, Nobuyuki Matsushima, Kyosuke Uno, Yukio Yoneda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the present study, we have attempted to evaluate the pharmacological actions of three major phenolic antidiarrheic ingredients, including 2-methoxyphenol (2MP), 2-methoxy-4-methylphenol (2M4MP) and 2-methoxy-4-ethyphenol (2M4EP), on the functionality and integrity of bone by in vitro and in vivo experimental techniques. Intermittent oral administration of 2M4MP and 2M4EP, but not 2MP, significantly prevented reductions of bone mineral density in total femur, distal femur and tibia, in addition to alterations of several osteoclastic parameters on histomorphometric analysis, when determined 28 days after ovariectomy in mice. All three phenolic ingredients examined significantly inhibited the developmental increase in the number of multinucleated cells positive to tartrate-resistant acid phosphatase staining in cultured mouse osteoclasts differentiated from bone marrow precursors in the presence of both macrophage-colony stimulating factor and receptor activator of nuclear factor-κB ligand, which occurred in a concentration-dependent manner at a concentration range of 1 μM-1 mM without inducing cell death. Moreover, both 2M4MP and 2M4EP at 1 mM not only prevented the cell death induced by 0.5 mM H2O2 in cultured rat calvarial osteoblasts, but also suppressed the generation of intracellular reactive oxygen species in osteoblasts exposed to H2O2, with a radical scavenging action as revealed by electron spin resonance analysis. These results suggest that particular phenolic antidiarrheic ingredients may prevent ovariectomy-induced bone loss through a mechanism related to the inhibition of osteoclastogenesis in association with an anti-oxidative property in osteoblasts.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalBiochemical Pharmacology
Volume73
Issue number3
DOIs
Publication statusPublished - Feb 1 2007
Externally publishedYes

Fingerprint

Ovariectomy
Osteoblasts
Guaiacol
Oral Administration
Bone
Bone and Bones
Femur
Cell Death
Cell death
Macrophage Colony-Stimulating Factor Receptors
Electron Spin Resonance Spectroscopy
Osteoclasts
Tibia
Osteogenesis
Bone Density
Reactive Oxygen Species
Scavenging
Acid Phosphatase
Cell Count
Bone Marrow

Keywords

  • Antidiarrheic
  • Bone loss
  • Osteoblasts
  • Osteoclasts
  • Ovariectomy

ASJC Scopus subject areas

  • Pharmacology

Cite this

Oral administration of phenolic antidiarrheic ingredients prevents ovariectomy-induced bone loss. / Moriguchi, Nobuaki; Hinoi, Eiichi; Takarada, Takeshi; Matsushima, Nobuyuki; Uno, Kyosuke; Yoneda, Yukio.

In: Biochemical Pharmacology, Vol. 73, No. 3, 01.02.2007, p. 385-393.

Research output: Contribution to journalArticle

Moriguchi, Nobuaki ; Hinoi, Eiichi ; Takarada, Takeshi ; Matsushima, Nobuyuki ; Uno, Kyosuke ; Yoneda, Yukio. / Oral administration of phenolic antidiarrheic ingredients prevents ovariectomy-induced bone loss. In: Biochemical Pharmacology. 2007 ; Vol. 73, No. 3. pp. 385-393.
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