Abstract
Fine W/O/W emulsions having controlled median diameters between 0.7 and 30 μm were prepared using octanoic acid triacylglycerol (C8:0TG) and soybean oil as the oil phases. Transport enhancement of a model hydrophilic compound by the soybean-oil hydrolysates was investigated using Caco-2 model intestinal epithelial cells. The results were compared with those from in vivo experiments; in which rats were administered various types of W/O/W emulsions encapsulating insulin. Transport enhancement effect of the emulsions was evaluated by the blood-sugar level collected from the coccygeal vein. The pancreatic lipase-catalyzed hydrolysates of soybean oil showed high transport enhancement effects in Caco-2 model system, but the administration of insulin included in the W/O/W emulsion prepared with soybean oil had no hypoglycemic effect on rats. The transport enhancement effect of the hydrolysates of soybean oil in Caco-2 model system was attenuated by the coexistence of a coating material used in spray-drying process or triacylglycerol. On the other hand, administration of the W/O/W emulsions prepared with C8:0TG and encapsulating insulin showed a hypoglycemic effect on rats. Administration of the W/O/W emulsion having an oil-droplet median diameter of 2.3 μm also showed the hypoglycemic effect in the earlier period than that of the coarse W/O/W emulsion having a diameter of 38 μm. However, the emulsion with the diameter of 0.7 μm exhibited no effect. These results suggested that the transport enhancement effect of the W/O/W emulsion prepared with C8:0TG depended on the size of the oil droplets, while too fine an emulsion had no such effect.
| Original language | English |
|---|---|
| Pages (from-to) | 523-531 |
| Number of pages | 9 |
| Journal | Food Hydrocolloids |
| Volume | 20 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jun 2006 |
| Externally published | Yes |
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Keywords
- Blood-sugar level
- Insulin
- Medium chain fatty acid triacylglycerol
- Rat
- Soybean oil
- Transport enhancement
- W/O/W emulsion
ASJC Scopus subject areas
- Food Science
Cite this
Oral administration of insulin included in fine W/O/W emulsions to rats. / Shima, Motohiro; Tanaka, Miyono; Fujii, Takao; Egawa, Kahori; Kimura, Yukitaka; Adachi, Shuji; Matsuno, Ryuichi.
In: Food Hydrocolloids, Vol. 20, No. 4, 06.2006, p. 523-531.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oral administration of insulin included in fine W/O/W emulsions to rats
AU - Shima, Motohiro
AU - Tanaka, Miyono
AU - Fujii, Takao
AU - Egawa, Kahori
AU - Kimura, Yukitaka
AU - Adachi, Shuji
AU - Matsuno, Ryuichi
PY - 2006/6
Y1 - 2006/6
N2 - Fine W/O/W emulsions having controlled median diameters between 0.7 and 30 μm were prepared using octanoic acid triacylglycerol (C8:0TG) and soybean oil as the oil phases. Transport enhancement of a model hydrophilic compound by the soybean-oil hydrolysates was investigated using Caco-2 model intestinal epithelial cells. The results were compared with those from in vivo experiments; in which rats were administered various types of W/O/W emulsions encapsulating insulin. Transport enhancement effect of the emulsions was evaluated by the blood-sugar level collected from the coccygeal vein. The pancreatic lipase-catalyzed hydrolysates of soybean oil showed high transport enhancement effects in Caco-2 model system, but the administration of insulin included in the W/O/W emulsion prepared with soybean oil had no hypoglycemic effect on rats. The transport enhancement effect of the hydrolysates of soybean oil in Caco-2 model system was attenuated by the coexistence of a coating material used in spray-drying process or triacylglycerol. On the other hand, administration of the W/O/W emulsions prepared with C8:0TG and encapsulating insulin showed a hypoglycemic effect on rats. Administration of the W/O/W emulsion having an oil-droplet median diameter of 2.3 μm also showed the hypoglycemic effect in the earlier period than that of the coarse W/O/W emulsion having a diameter of 38 μm. However, the emulsion with the diameter of 0.7 μm exhibited no effect. These results suggested that the transport enhancement effect of the W/O/W emulsion prepared with C8:0TG depended on the size of the oil droplets, while too fine an emulsion had no such effect.
AB - Fine W/O/W emulsions having controlled median diameters between 0.7 and 30 μm were prepared using octanoic acid triacylglycerol (C8:0TG) and soybean oil as the oil phases. Transport enhancement of a model hydrophilic compound by the soybean-oil hydrolysates was investigated using Caco-2 model intestinal epithelial cells. The results were compared with those from in vivo experiments; in which rats were administered various types of W/O/W emulsions encapsulating insulin. Transport enhancement effect of the emulsions was evaluated by the blood-sugar level collected from the coccygeal vein. The pancreatic lipase-catalyzed hydrolysates of soybean oil showed high transport enhancement effects in Caco-2 model system, but the administration of insulin included in the W/O/W emulsion prepared with soybean oil had no hypoglycemic effect on rats. The transport enhancement effect of the hydrolysates of soybean oil in Caco-2 model system was attenuated by the coexistence of a coating material used in spray-drying process or triacylglycerol. On the other hand, administration of the W/O/W emulsions prepared with C8:0TG and encapsulating insulin showed a hypoglycemic effect on rats. Administration of the W/O/W emulsion having an oil-droplet median diameter of 2.3 μm also showed the hypoglycemic effect in the earlier period than that of the coarse W/O/W emulsion having a diameter of 38 μm. However, the emulsion with the diameter of 0.7 μm exhibited no effect. These results suggested that the transport enhancement effect of the W/O/W emulsion prepared with C8:0TG depended on the size of the oil droplets, while too fine an emulsion had no such effect.
KW - Blood-sugar level
KW - Insulin
KW - Medium chain fatty acid triacylglycerol
KW - Rat
KW - Soybean oil
KW - Transport enhancement
KW - W/O/W emulsion
UR - http://www.scopus.com/inward/record.url?scp=32244435946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=32244435946&partnerID=8YFLogxK
U2 - 10.1016/j.foodhyd.2005.05.002
DO - 10.1016/j.foodhyd.2005.05.002
M3 - Article
AN - SCOPUS:32244435946
VL - 20
SP - 523
EP - 531
JO - Food Hydrocolloids
JF - Food Hydrocolloids
SN - 0268-005X
IS - 4
ER -