Oral administration of insulin included in fine W/O/W emulsions to rats

Motohiro Shima, Miyono Tanaka, Takao Fujii, Kahori Egawa, Yukitaka Kimura, Shuji Adachi, Ryuichi Matsuno

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Fine W/O/W emulsions having controlled median diameters between 0.7 and 30 μm were prepared using octanoic acid triacylglycerol (C8:0TG) and soybean oil as the oil phases. Transport enhancement of a model hydrophilic compound by the soybean-oil hydrolysates was investigated using Caco-2 model intestinal epithelial cells. The results were compared with those from in vivo experiments; in which rats were administered various types of W/O/W emulsions encapsulating insulin. Transport enhancement effect of the emulsions was evaluated by the blood-sugar level collected from the coccygeal vein. The pancreatic lipase-catalyzed hydrolysates of soybean oil showed high transport enhancement effects in Caco-2 model system, but the administration of insulin included in the W/O/W emulsion prepared with soybean oil had no hypoglycemic effect on rats. The transport enhancement effect of the hydrolysates of soybean oil in Caco-2 model system was attenuated by the coexistence of a coating material used in spray-drying process or triacylglycerol. On the other hand, administration of the W/O/W emulsions prepared with C8:0TG and encapsulating insulin showed a hypoglycemic effect on rats. Administration of the W/O/W emulsion having an oil-droplet median diameter of 2.3 μm also showed the hypoglycemic effect in the earlier period than that of the coarse W/O/W emulsion having a diameter of 38 μm. However, the emulsion with the diameter of 0.7 μm exhibited no effect. These results suggested that the transport enhancement effect of the W/O/W emulsion prepared with C8:0TG depended on the size of the oil droplets, while too fine an emulsion had no such effect.

Original languageEnglish
Pages (from-to)523-531
Number of pages9
JournalFood Hydrocolloids
Volume20
Issue number4
DOIs
Publication statusPublished - Jun 2006
Externally publishedYes

Fingerprint

Insulin
Emulsions
oral administration
emulsions
Oral Administration
Rats
insulin
Soybean oil
Soybean Oil
rats
soybean oil
glycemic effect
hydrolysates
Hypoglycemic Agents
Oils
oils
droplets
Triglycerides
triacylglycerols
octanoic acid

Keywords

  • Blood-sugar level
  • Insulin
  • Medium chain fatty acid triacylglycerol
  • Rat
  • Soybean oil
  • Transport enhancement
  • W/O/W emulsion

ASJC Scopus subject areas

  • Food Science

Cite this

Shima, M., Tanaka, M., Fujii, T., Egawa, K., Kimura, Y., Adachi, S., & Matsuno, R. (2006). Oral administration of insulin included in fine W/O/W emulsions to rats. Food Hydrocolloids, 20(4), 523-531. https://doi.org/10.1016/j.foodhyd.2005.05.002

Oral administration of insulin included in fine W/O/W emulsions to rats. / Shima, Motohiro; Tanaka, Miyono; Fujii, Takao; Egawa, Kahori; Kimura, Yukitaka; Adachi, Shuji; Matsuno, Ryuichi.

In: Food Hydrocolloids, Vol. 20, No. 4, 06.2006, p. 523-531.

Research output: Contribution to journalArticle

Shima, M, Tanaka, M, Fujii, T, Egawa, K, Kimura, Y, Adachi, S & Matsuno, R 2006, 'Oral administration of insulin included in fine W/O/W emulsions to rats', Food Hydrocolloids, vol. 20, no. 4, pp. 523-531. https://doi.org/10.1016/j.foodhyd.2005.05.002
Shima, Motohiro ; Tanaka, Miyono ; Fujii, Takao ; Egawa, Kahori ; Kimura, Yukitaka ; Adachi, Shuji ; Matsuno, Ryuichi. / Oral administration of insulin included in fine W/O/W emulsions to rats. In: Food Hydrocolloids. 2006 ; Vol. 20, No. 4. pp. 523-531.
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