Optogenetic regulation of embryo implantation in mice using photoactivatable CRISPR-Cas9

Tomoka Takao, Moritoshi Sato, Tetsuo Maruyama

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Embryo implantation is achieved upon successful interaction between a fertilized egg and receptive endometrium and is mediated by spatiotemporal expression of implantation-associated molecules including leukemia inhibitory factor (LIF). Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPRCas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility. This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation and provides a therapeutic strategy for temporal control of reproductive functions in vivo.

Original languageEnglish
Pages (from-to)28579-28581
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number46
DOIs
Publication statusPublished - Nov 17 2020

Keywords

  • Implantation
  • Leukemia inhibitory factor
  • Optogenetic
  • Photoactivatable CRISPR-Cas9

ASJC Scopus subject areas

  • General

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