Optimum force and duration of toothbrusing to enhance gingival fibroblast proliferation and procollagen type I synthesis in dogs

Takaaki Tomofuji, Daisuke Ekuni, Tatsuo Yamamoto, Masazumi Horiuchi, Tomonori Sakamoto, Tatsuo Watanabe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Toothbrushing enhances gingival fibroblast proliferation, which promotes wound healing. Optimum force and duration of toothbrushing for stimulation of fibroblast proliferation are key factors in maximizing effects of toothbrushing on periodontal wound healing. We therefore evaluated the effects of different durations and forces of toothbrushing on proliferative activity and procollagen synthesis of gingival fibroblasts. Methods: Twelve dogs were used. In each dog, buccal gingivae of 12 teeth were examined for 3 weeks. Nine of these 12 teeth were each assigned to 1 of 9 different combinations of brushing force (0.98, 1.96, or 2.45 N) and duration (10, 20, or 40 seconds). The remaining 3 teeth received plaque removal without brushing, via a scaler. Results: Force and duration of toothbrushing affected both proliferating cell nuclear antigen (PCNA)-positive and procollagen Type I C-peptide (PIP)-positive fibroblast ratios (P <0.05). The highest ratio of PCNA-positive fibroblasts was produced by brushing at 1.96 N for 20 seconds. The highest ratio of PIP-positive fibroblasts was produced by brushing at 1.96 N for 10 seconds. Conclusions: Toothbrushing at certain forces and durations enhanced the proliferative activity and procollagen synthesis of gingival fibroblasts. The toothbrushing duration that increased procollagen synthesis (10 seconds) was shorter than that which increased fibroblast proliferative activity (20 seconds).

Original languageEnglish
Pages (from-to)630-634
Number of pages5
JournalJournal of periodontology
Volume74
Issue number5
DOIs
Publication statusPublished - May 1 2003

Keywords

  • Fibroblasts
  • Gingival/growth and development
  • Procollagen
  • Proliferating cell nuclear antigen
  • Toothbrushing
  • Wound healing

ASJC Scopus subject areas

  • Periodontics

Fingerprint Dive into the research topics of 'Optimum force and duration of toothbrusing to enhance gingival fibroblast proliferation and procollagen type I synthesis in dogs'. Together they form a unique fingerprint.

  • Cite this