Optimization of bacterial plasmid transformation using nanomaterials based on the Yoshida effect

Haidong Tan, Li Fu, Masaharu Seno

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.

Original languageEnglish
Pages (from-to)4962-4972
Number of pages11
JournalInternational Journal of Molecular Sciences
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Bacterial Transformation
Calcium chloride
plasmids
Nanostructures
Nanostructured materials
Carbon nanotubes
Bacteria
Plasmids
DNA
Calcium Chloride
Carbon Nanotubes
optimization
calcium chlorides
bacteria
Electroporation
deoxyribonucleic acid
carbon nanotubes

Keywords

  • Carbon nanotube
  • Nanomaterials
  • Plasmid transformation
  • Sepiolite
  • Yoshida effect

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Medicine(all)

Cite this

Optimization of bacterial plasmid transformation using nanomaterials based on the Yoshida effect. / Tan, Haidong; Fu, Li; Seno, Masaharu.

In: International Journal of Molecular Sciences, Vol. 11, No. 12, 12.2010, p. 4962-4972.

Research output: Contribution to journalArticle

@article{2eeb53ddcf5a47328afabc4f0031d7bf,
title = "Optimization of bacterial plasmid transformation using nanomaterials based on the Yoshida effect",
abstract = "With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.",
keywords = "Carbon nanotube, Nanomaterials, Plasmid transformation, Sepiolite, Yoshida effect",
author = "Haidong Tan and Li Fu and Masaharu Seno",
year = "2010",
month = "12",
doi = "10.3390/ijms11124962",
language = "English",
volume = "11",
pages = "4962--4972",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",

}

TY - JOUR

T1 - Optimization of bacterial plasmid transformation using nanomaterials based on the Yoshida effect

AU - Tan, Haidong

AU - Fu, Li

AU - Seno, Masaharu

PY - 2010/12

Y1 - 2010/12

N2 - With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.

AB - With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.

KW - Carbon nanotube

KW - Nanomaterials

KW - Plasmid transformation

KW - Sepiolite

KW - Yoshida effect

UR - http://www.scopus.com/inward/record.url?scp=78650968362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650968362&partnerID=8YFLogxK

U2 - 10.3390/ijms11124962

DO - 10.3390/ijms11124962

M3 - Article

C2 - 21614185

AN - SCOPUS:78650968362

VL - 11

SP - 4962

EP - 4972

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 12

ER -