Optimization of bacterial plasmid transformation using nanomaterials based on the Yoshida effect

Haidong Tan, Li Fu, Masaharu Seno

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.

Original languageEnglish
Pages (from-to)4962-4972
Number of pages11
JournalInternational journal of molecular sciences
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 2010

Keywords

  • Carbon nanotube
  • Nanomaterials
  • Plasmid transformation
  • Sepiolite
  • Yoshida effect

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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