Opposite effects of isoniazid and fasting on the expression of CYP2E1 protein and mRNA in house musk shrew (Suncus murinus)

Hironori Nakura, Susumu Itoh, Hisashi Kusano, Hiroyuki Ishizone, Tetsuya Kamataki

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The effects of isoniazid and fasting on hepatic CYP2E1 in the suncus were investigated. Aniline hydroxylation and N-nitrosodimethylamine demethylation, which are known to be catalyzed by CYP2E1, in liver microsomes were induced and suppressed by the treatment with isoniazid and the fasting, respectively. Immunoblot analysis indicated that CYP2E1 protein in liver microsomes from isoniazid-treated suncus was increased in contrast to the result with the fasting of the suncus. Northern blot analysis showed that the treatment of suncus with isoniazid increased the expression of CYP2E1 mRNA in livers although the fasting of the suncus significantly decreased CYP2E1 mRNA. These results suggest that the regulation of hepatic CYP2E1 in the suncus by treatment with isoniazid and fasting was different from that in rats.

Original languageEnglish
Pages (from-to)293-301
Number of pages9
JournalBiochemistry and Molecular Biology International
Issue number2
Publication statusPublished - Feb 1 1997
Externally publishedYes



  • CYP2E1
  • Fasting
  • INH-treatment
  • Suncus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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