Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor

Keita Sato, Takahiro Yamashita, Hideyo Ohuchi, Atsuko Takeuchi, Hitoshi Gotoh, Katsuhiko Ono, Misao Mizuno, Yasuhisa Mizutani, Sayuri Tomonari, Kazumi Sakai, Yasushi Imamoto, Akimori Wada, Yoshinori Shichida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Most opsins are G protein-coupled receptors that utilize retinal both as a ligand and as a chromophore. Opsins' main established mechanism is light-triggered activation through retinal 11-cis-to-all-trans photoisomerization. Here we report a vertebrate non-visual opsin that functions as a Gi-coupled retinal receptor that is deactivated by light and can thermally self-regenerate. This opsin, Opn5L1, binds exclusively to all-trans-retinal. More interestingly, the light-induced deactivation through retinal trans-to-cis isomerization is followed by formation of a covalent adduct between retinal and a nearby cysteine, which breaks the retinal-conjugated double bond system, probably at the C11 position, resulting in thermal re-isomerization to all-trans-retinal. Thus, Opn5L1 acts as a reverse photoreceptor. We conclude that, like vertebrate rhodopsin, Opn5L1 is a unidirectional optical switch optimized from an ancestral bidirectional optical switch, such as invertebrate rhodopsin, to increase the S/N ratio of the signal transduction, although the direction of optimization is opposite to that of vertebrate rhodopsin.

Original languageEnglish
Article number1255
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Opsins
photoreceptors
vertebrates
Rhodopsin
Vertebrates
isomerization
Optical switches
switches
Isomerization
Light
invertebrates
cysteine
Retinaldehyde
Photoisomerization
deactivation
Signal transduction
Retinal Perforations
chromophores
adducts
Invertebrates

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor. / Sato, Keita; Yamashita, Takahiro; Ohuchi, Hideyo; Takeuchi, Atsuko; Gotoh, Hitoshi; Ono, Katsuhiko; Mizuno, Misao; Mizutani, Yasuhisa; Tomonari, Sayuri; Sakai, Kazumi; Imamoto, Yasushi; Wada, Akimori; Shichida, Yoshinori.

In: Nature Communications, Vol. 9, No. 1, 1255, 01.12.2018.

Research output: Contribution to journalArticle

Sato, K, Yamashita, T, Ohuchi, H, Takeuchi, A, Gotoh, H, Ono, K, Mizuno, M, Mizutani, Y, Tomonari, S, Sakai, K, Imamoto, Y, Wada, A & Shichida, Y 2018, 'Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor', Nature Communications, vol. 9, no. 1, 1255. https://doi.org/10.1038/s41467-018-03603-3
Sato, Keita ; Yamashita, Takahiro ; Ohuchi, Hideyo ; Takeuchi, Atsuko ; Gotoh, Hitoshi ; Ono, Katsuhiko ; Mizuno, Misao ; Mizutani, Yasuhisa ; Tomonari, Sayuri ; Sakai, Kazumi ; Imamoto, Yasushi ; Wada, Akimori ; Shichida, Yoshinori. / Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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