Operon structure of flagellar genes in Salmonella typhimurium

Kazuhiro Kutsukake; Yoshikazu Ohya; Shigeru Yamaguchi; Tetsuo Iino

doi:10.1007/BF00340172

Operon structure of flagellar genes in Salmonella typhimurium

Kazuhiro Kutsukake, Yoshikazu Ohya, Shigeru Yamaguchi, Tetsuo Iino

Graduate School of Natural Science and Technology

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

In Salmonella typhimurium, more than 40 genes have been shown to be involved in flagellar formation and function and almost all of them have been assigned to three regions of the chromosome, termed region I, region II, and region III. In the present study, a large number of transposon-insertion mutants in these flagellar genes were isolated using Tn 10 and Mu d1. The flaV gene was found to be a strong hot spot for Tn 10 insertion. Complementation analysis of the polarity effects exerted by the transposon-insertion mutants defined 13 different flagellar operons; 3 in region I, 4 in region II, and 6 in region III. These results are compared with the reported arrangement of the corresponding genes in Escherichia coli.

Original language	English
Pages (from-to)	11-15
Number of pages	5
Journal	MGG Molecular & General Genetics
Volume	214
Issue number	1
DOIs	https://doi.org/10.1007/BF00340172
Publication status	Published - Sep 1 1988

Keywords

Complementation analysis
Flagellar genes
Flagellar operons
Polar effect
Transposon

ASJC Scopus subject areas

Genetics

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10.1007/BF00340172

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title = "Operon structure of flagellar genes in Salmonella typhimurium",

abstract = "In Salmonella typhimurium, more than 40 genes have been shown to be involved in flagellar formation and function and almost all of them have been assigned to three regions of the chromosome, termed region I, region II, and region III. In the present study, a large number of transposon-insertion mutants in these flagellar genes were isolated using Tn 10 and Mu d1. The flaV gene was found to be a strong hot spot for Tn 10 insertion. Complementation analysis of the polarity effects exerted by the transposon-insertion mutants defined 13 different flagellar operons; 3 in region I, 4 in region II, and 6 in region III. These results are compared with the reported arrangement of the corresponding genes in Escherichia coli.",

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T1 - Operon structure of flagellar genes in Salmonella typhimurium

AU - Kutsukake, Kazuhiro

AU - Ohya, Yoshikazu

AU - Yamaguchi, Shigeru

AU - Iino, Tetsuo

PY - 1988/9/1

Y1 - 1988/9/1

N2 - In Salmonella typhimurium, more than 40 genes have been shown to be involved in flagellar formation and function and almost all of them have been assigned to three regions of the chromosome, termed region I, region II, and region III. In the present study, a large number of transposon-insertion mutants in these flagellar genes were isolated using Tn 10 and Mu d1. The flaV gene was found to be a strong hot spot for Tn 10 insertion. Complementation analysis of the polarity effects exerted by the transposon-insertion mutants defined 13 different flagellar operons; 3 in region I, 4 in region II, and 6 in region III. These results are compared with the reported arrangement of the corresponding genes in Escherichia coli.

AB - In Salmonella typhimurium, more than 40 genes have been shown to be involved in flagellar formation and function and almost all of them have been assigned to three regions of the chromosome, termed region I, region II, and region III. In the present study, a large number of transposon-insertion mutants in these flagellar genes were isolated using Tn 10 and Mu d1. The flaV gene was found to be a strong hot spot for Tn 10 insertion. Complementation analysis of the polarity effects exerted by the transposon-insertion mutants defined 13 different flagellar operons; 3 in region I, 4 in region II, and 6 in region III. These results are compared with the reported arrangement of the corresponding genes in Escherichia coli.

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