Open stomata 1 kinase is essential for yeast elicitor-induced stomatal closure in arabidopsis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We recently demonstrated that yeast elicitor (YEL)-induced stomatal closure requires a Ca2+-dependent kinase, CPK6. A Ca2+-independent kinase, Open Stomata 1 (OST1), is involved in stomatal closure induced by various stimuli including ABA. In the present study, we investigated the role of OST1 in YEL-induced stomatal closure in Arabidopsis using a knock-out mutant, ost1-3, and a kinase-deficient mutant, ost1-2. YEL did not induce stomatal closure or activation of guard cell S-type anion channels in the ost1 mutants unlike in wild-type plants. However, YEL did not increase OST1 kinase activity in wild-type guard cells. The YEL-induced stomatal closure and activation of S-type anion channels were also impaired in a gain-of-function mutant of a clade A type 2C protein phosphatase (ABA INSENSITIVE 1), abi1-1C. In the ost1 mutants like in the wild type, YEL induced H2O2 accumulation, activation of non-selective Ca2+-permeable cation (ICa) channels and transient elevations in cytosolic free Ca2+ concentration ([Ca2+]cyt) in guard cells. These results suggest that OST1 kinase is essential for stomatal closure and activation of S-type anion channels induced by YEL and that OST1 is not involved in H2O2 accumulation, ICa channel activation or [Ca2+]cyt elevations in guard cells induced by YEL.

Original languageEnglish
Pages (from-to)1239-1248
Number of pages10
JournalPlant and Cell Physiology
Volume56
Issue number6
DOIs
Publication statusPublished - Dec 22 2014

Fingerprint

Arabidopsis
stomata
phosphotransferases (kinases)
Phosphotransferases
Yeasts
yeasts
guard cells
calcium
anions
Anions
mutants
abscisic acid
knockout mutants
elicitors
Cations
cations

Keywords

  • Arabidopsis
  • Calcium signaling
  • Guard cell
  • Open Stomata 1
  • S-type anion channel
  • Yeast elicitor

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Open stomata 1 kinase is essential for yeast elicitor-induced stomatal closure in arabidopsis. / Ye, Wenxiu; Adachi, Yuji; Munemasa, Shintaro; Nakamura, Yoshimasa; Mori, Izumi; Murata, Yoshiyuki.

In: Plant and Cell Physiology, Vol. 56, No. 6, 22.12.2014, p. 1239-1248.

Research output: Contribution to journalArticle

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