Oocyte transport

Developmental competence of bovine oocytes arrested at germinal vesicle stage by cycloheximide under air

Shu Hashimoto, Koji Kimura, Hisataka Iwata, Ryo Takakura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effects of the medium (TCM 199 or SOFaa) and temperature (20 or 39 C) during meiotic arrest by cycloheximide (CHX) under air on the developmental competence of bovine oocytes after in vitro maturation (IVM) and fertilization (IVF) were investigated. Oocytes were maintained in meiotic arrest by 10 μg/ml CHX in a 50-μl droplet of 25-mM HEPES-buffered TCM 199 (H199) at 39 C or synthetic oviduct fluid (HSOFaa) at 20 or 39 C in air for 24 h. After release from the arrest, the oocytes was matured and fertilized in vitro and their developmental competence was examined. The developmental rate of oocytes arrested in HSOFaa at 20 C to the blastocyst stage was similar to that of non-arrested oocytes but was significantly higher (P

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalJournal of Reproduction and Development
Volume49
Issue number1
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

germinal vesicle
cycloheximide
Cycloheximide
Mental Competency
Oocytes
oocytes
Air
air
cattle
In Vitro Oocyte Maturation Techniques
HEPES
Oviducts
Blastocyst
Fertilization in Vitro
oviducts
blastocyst
droplets
Temperature
temperature

Keywords

  • Bovine oocyte
  • Cycloheximide
  • Meiotic arrest

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Oocyte transport : Developmental competence of bovine oocytes arrested at germinal vesicle stage by cycloheximide under air. / Hashimoto, Shu; Kimura, Koji; Iwata, Hisataka; Takakura, Ryo.

In: Journal of Reproduction and Development, Vol. 49, No. 1, 2003, p. 61-66.

Research output: Contribution to journalArticle

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