Onion root water transport sensitive to water channel and K+ channel inhibitors

Masashi Tazawa, Emi Sutou, Mineo Shibasaka

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Transroot osmotic water flux (Jos) and radial hydraulic conductivity (Lpr) in onion roots were greatly increased by three means; infiltration of roots by pressurization, repetition of osmosis and chilling at 5°C. Jos was strongly reduced by the water channel inhibitor HgCl2 (91%) and the K+ channel inhibitor nonyltriethylammonium (C9, 75%), which actually made the membrane potential of root cells less sensitive to K+. C9decreased the rate of turgor reduction induced by sorbitol solution to the same extent as HgCl2. Thus, C9 is assumed to decrease the hydraulic conductivity (Lp) of the plasma membrane by blocking water channels, although possible inhibition of the plasmodesmata of the root symplast by C9 cannot be excluded. Onion roots transported water from the tip to the base in the absence of the osmotic gradient. This non-osmotic water flux (Jnos) was equivalent to Jos induced by 0.029 M sorbitol. Jnos increased when Jos was increased by repetition of osmosis and decreased when Jos was decreased by either HgCl2 or by C9. The correlation between Jnos and Jos suggests that non-osmotic water transport occurs via the same pathways as those for osmotic water transport.

Original languageEnglish
Pages (from-to)28-36
Number of pages9
JournalPlant and Cell Physiology
Volume42
Issue number1
Publication statusPublished - 2001

Fingerprint

Aquaporins
Onions
potassium channels
onions
Mercuric Chloride
Water
Osmosis
Sorbitol
osmosis
water
Plasmodesmata
sorbitol
hydraulic conductivity
Membrane Potentials
symplast
plasmodesmata
turgor
Cell Membrane
membrane potential
infiltration (hydrology)

Keywords

  • Hydraulic conductivity
  • K channel inhibitor
  • Membrane potential
  • Non-osmotic water transport
  • Onion roots
  • Water channel inhibitor

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Onion root water transport sensitive to water channel and K+ channel inhibitors. / Tazawa, Masashi; Sutou, Emi; Shibasaka, Mineo.

In: Plant and Cell Physiology, Vol. 42, No. 1, 2001, p. 28-36.

Research output: Contribution to journalArticle

Tazawa, M, Sutou, E & Shibasaka, M 2001, 'Onion root water transport sensitive to water channel and K+ channel inhibitors', Plant and Cell Physiology, vol. 42, no. 1, pp. 28-36.
Tazawa, Masashi ; Sutou, Emi ; Shibasaka, Mineo. / Onion root water transport sensitive to water channel and K+ channel inhibitors. In: Plant and Cell Physiology. 2001 ; Vol. 42, No. 1. pp. 28-36.
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