On-chip real-time PCR for genetic tug-of-war (g-TOW) experiment

Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Yukihiro Okamoto, Manabu Tokeshi, Yosinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A micro-chip based real-time PCR device has been developed for biorobustness research and systems biology. The device is constructed of a silicon-glass chip, a Peltier element and a microscopy. With the device, amplification of target gene on a plasmid and housekeeping gene were detected simultaneously, and the copy number of the plasmid could be estimated. We had optimized the device, and achieved detection of fluorescent intensity increase in reaction channels.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages627-629
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Keywords

  • Real-time PCR
  • Silicon chip
  • Systems biology

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Naito, T., Yatsuhashi, A., Kaji, N., Ando, T., Sato, K., Moriya, H., Kitano, H., Okamoto, Y., Tokeshi, M., & Baba, Y. (2009). On-chip real-time PCR for genetic tug-of-war (g-TOW) experiment. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 627-629). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.