Olive fruit debittering significantly alters its antioxidant activity as evaluated with multiple free-radical scavenging ability

Yoshimi Sueishi, Risako Nii

Research output: Contribution to journalArticlepeer-review

Abstract

Raw olive fruits taste bitter and are not edible without debittering. Common debittering process promotes the loss of olive antioxidant activity and the magnitude of loss depends on the debittering reagent used. Antioxidant activity was evaluated as the scavenging ability that was determined with multiple free-radical scavenging (MULTIS) method, where the scavenging abilities against five reactive oxygen species (HO·, O2·, RO·, t-BuOO·, and 1O2) were determined. Significant change in the scavenging activity of debittered olive was found in four debittering methods, i.e., sodium hydroxide, sodium hydrogen carbonate, ethanol, and sodium chloride treatments. Results indicated that the scavenging activities lowered by 60–90% after most debittering processes. Sodium chloride treatment least affected the scavenging activity. Based on the MULTIS measurements for the antioxidant components and debittering reagents, the rate constants of antioxidant compounds with four ROS were determined and the antioxidant activity that depends on the method of debittering is discussed. Oleuropein, olive-related antioxidant compound, showed high scavenging abilities against HO· (k = 6.70 × 109 M−1 s−1), RO· (k = 4.69 × 106 M−1 s−1), and 1O2 (k = 1.74 × 107 M−1 s−1). This study suggests that functionality and taste do not always come together. MULTIS method is useful in comparative antioxidant capacity studies in foods.

Original languageEnglish
Pages (from-to)1403-1409
Number of pages7
JournalJournal of Food Measurement and Characterization
Volume15
Issue number2
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Antioxidant capacity
  • Debittering treatment
  • ESR spin trapping
  • MULTIS
  • Olive

ASJC Scopus subject areas

  • Food Science
  • Chemical Engineering(all)
  • Safety, Risk, Reliability and Quality
  • Industrial and Manufacturing Engineering

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