Oligonucleotide array analysis of cyclic tension-responsive genes in human periodontal ligament fibroblasts

Keisuke Yamashiro, Fumio Myokai, Koichi Hiratsuka, Tadashi Yamamoto, Kyoko Senoo, Hideo Arai, Fusanori Nishimura, Yoshimitsu Abiko, Shogo Takashiba

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mechanical stress results in differential gene expression that is critical to convert the stimulus into biochemical signals. Under physiological stress such as occlusal force, human periodontal ligament fibroblasts (HPLF) are associated with homeostasis of periodontal tissues however the changes in response to mechanotransduction remain uncharacterized. We hypothesized that cyclic tension-responsive (CT) genes may be used to identify a set of fundamental pathways of mechanotransduction. Our goal was to catalogue CT genes in cultured HPLF. HPLF were subjected to cyclic tension up to 16 h, and total RNA was isolated from both tension-loaded and static HPLF. The oligonucleotide arrays analysis revealed significant changes of mRNA accumulation for 122 CT genes, and their kinetics were assigned by the K-means clustering methods. Ingenuity Pathway Analysis was completed for HPLF mechanotransduction using 50 CT genes. This analysis revealed that cyclic tension immediately down-regulated all nuclear transcription factors except v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS) reacting as an early responsive gene. In turn, transcription factors such as tumor protein p53 binding protein 2 (TP53BP2), and extra-nuclear molecules such as adrenergic receptor β2 (ADRB2) were up-regulated after 1-2 h, which may result in fundamental HPLF functions to adapt to cyclic tension. Subsequent inhibition assays using Y27632, a pharmacologic inhibitor of Rho-associated kinase (ROCK), suggested that HPLF has both ROCK-dependent and ROCK-independent CT genes. Mechanical stress was found to effect the expression of numerous genes, in particular, expression of an early responsive gene; FOS initiates alteration of HPLF behaviors to control homeostasis of the periodontal ligament.

Original languageEnglish
Pages (from-to)910-921
Number of pages12
JournalInternational Journal of Biochemistry and Cell Biology
Volume39
Issue number5
DOIs
Publication statusPublished - 2007

Fingerprint

Periodontal Ligament
Ligaments
Fourier Analysis
Fibroblasts
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Genes
rho-Associated Kinases
Mechanical Stress
Homeostasis
Transcription Factors
Bite Force
Gene Expression
Physiological Stress
Behavior Control
Osteosarcoma
Oncogenes
Protein Binding
Gene expression
Adrenergic Receptors

Keywords

  • Cyclic tension-responsive genes
  • Human periodontal ligament fibroblasts
  • Oligonucleotide array
  • Pathway analysis
  • Rho-associated kinase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Oligonucleotide array analysis of cyclic tension-responsive genes in human periodontal ligament fibroblasts. / Yamashiro, Keisuke; Myokai, Fumio; Hiratsuka, Koichi; Yamamoto, Tadashi; Senoo, Kyoko; Arai, Hideo; Nishimura, Fusanori; Abiko, Yoshimitsu; Takashiba, Shogo.

In: International Journal of Biochemistry and Cell Biology, Vol. 39, No. 5, 2007, p. 910-921.

Research output: Contribution to journalArticle

Yamashiro, Keisuke ; Myokai, Fumio ; Hiratsuka, Koichi ; Yamamoto, Tadashi ; Senoo, Kyoko ; Arai, Hideo ; Nishimura, Fusanori ; Abiko, Yoshimitsu ; Takashiba, Shogo. / Oligonucleotide array analysis of cyclic tension-responsive genes in human periodontal ligament fibroblasts. In: International Journal of Biochemistry and Cell Biology. 2007 ; Vol. 39, No. 5. pp. 910-921.
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