Olfactory tissue and regeneration medicine

Research output: Contribution to journalArticle

Abstract

Regeneration medicine is divided mainly into differentiation-induced and cell replenishment therapy. Bone marrow includes stem cell populations such as hematopoietic, mesenchymal and so on. These stem cells are shown experimentally and clinically to be pluripotent. It remains unclear, however, whether bone-marrow-derived cells differentiate into olfactory neurons and are engrafted as olfactory stem cells into olfactory tissue. We transplanted bone marrow cells of green fluorescence protein (GFP) mice into lethally irradiated recipients. Double immunostaining for GFP and olfactory marker protein or cytokeratin showed the possibility that bone-marrow-derived cell population could do as hoped. Because the engraftment rates of this study were low far from a practical level, methimazole inducing selectively apoptosis into olfactory tissue and granulocyte colony-stimulating factor known to mobilize stem cells from bone marrow into circulation were added to a series of experiments, which increased engraftment rates. Whether bone-marrow-derived cells or mesenchymal stem cells differentiate into olfactory cells remains controversial, necessitating the evaluation of regeneration medicine using such cells in further olfactory disorder studies.

Original languageEnglish
Pages (from-to)309-315
Number of pages7
JournalPractica Otologica
Volume104
Issue number5
DOIs
Publication statusPublished - May 2011

Fingerprint

Bone Marrow Cells
Regeneration
Stem Cells
Medicine
Olfactory Marker Protein
Fluorescence
Bone Marrow
Methimazole
Granulocyte Colony-Stimulating Factor
Cell- and Tissue-Based Therapy
Keratins
Mesenchymal Stromal Cells
Population
Proteins
Apoptosis
Neurons

Keywords

  • Bone marrow
  • Olfactory
  • Regeneration medicine
  • Transplantation

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Olfactory tissue and regeneration medicine. / Nishizaki, Kazunori.

In: Practica Otologica, Vol. 104, No. 5, 05.2011, p. 309-315.

Research output: Contribution to journalArticle

@article{eb09e75295044b36a4e663142507390f,
title = "Olfactory tissue and regeneration medicine",
abstract = "Regeneration medicine is divided mainly into differentiation-induced and cell replenishment therapy. Bone marrow includes stem cell populations such as hematopoietic, mesenchymal and so on. These stem cells are shown experimentally and clinically to be pluripotent. It remains unclear, however, whether bone-marrow-derived cells differentiate into olfactory neurons and are engrafted as olfactory stem cells into olfactory tissue. We transplanted bone marrow cells of green fluorescence protein (GFP) mice into lethally irradiated recipients. Double immunostaining for GFP and olfactory marker protein or cytokeratin showed the possibility that bone-marrow-derived cell population could do as hoped. Because the engraftment rates of this study were low far from a practical level, methimazole inducing selectively apoptosis into olfactory tissue and granulocyte colony-stimulating factor known to mobilize stem cells from bone marrow into circulation were added to a series of experiments, which increased engraftment rates. Whether bone-marrow-derived cells or mesenchymal stem cells differentiate into olfactory cells remains controversial, necessitating the evaluation of regeneration medicine using such cells in further olfactory disorder studies.",
keywords = "Bone marrow, Olfactory, Regeneration medicine, Transplantation",
author = "Kazunori Nishizaki",
year = "2011",
month = "5",
doi = "10.5631/jibirin.104.309",
language = "English",
volume = "104",
pages = "309--315",
journal = "Practica Otologica",
issn = "0032-6313",
publisher = "Society of Practical Otolaryngology",
number = "5",

}

TY - JOUR

T1 - Olfactory tissue and regeneration medicine

AU - Nishizaki, Kazunori

PY - 2011/5

Y1 - 2011/5

N2 - Regeneration medicine is divided mainly into differentiation-induced and cell replenishment therapy. Bone marrow includes stem cell populations such as hematopoietic, mesenchymal and so on. These stem cells are shown experimentally and clinically to be pluripotent. It remains unclear, however, whether bone-marrow-derived cells differentiate into olfactory neurons and are engrafted as olfactory stem cells into olfactory tissue. We transplanted bone marrow cells of green fluorescence protein (GFP) mice into lethally irradiated recipients. Double immunostaining for GFP and olfactory marker protein or cytokeratin showed the possibility that bone-marrow-derived cell population could do as hoped. Because the engraftment rates of this study were low far from a practical level, methimazole inducing selectively apoptosis into olfactory tissue and granulocyte colony-stimulating factor known to mobilize stem cells from bone marrow into circulation were added to a series of experiments, which increased engraftment rates. Whether bone-marrow-derived cells or mesenchymal stem cells differentiate into olfactory cells remains controversial, necessitating the evaluation of regeneration medicine using such cells in further olfactory disorder studies.

AB - Regeneration medicine is divided mainly into differentiation-induced and cell replenishment therapy. Bone marrow includes stem cell populations such as hematopoietic, mesenchymal and so on. These stem cells are shown experimentally and clinically to be pluripotent. It remains unclear, however, whether bone-marrow-derived cells differentiate into olfactory neurons and are engrafted as olfactory stem cells into olfactory tissue. We transplanted bone marrow cells of green fluorescence protein (GFP) mice into lethally irradiated recipients. Double immunostaining for GFP and olfactory marker protein or cytokeratin showed the possibility that bone-marrow-derived cell population could do as hoped. Because the engraftment rates of this study were low far from a practical level, methimazole inducing selectively apoptosis into olfactory tissue and granulocyte colony-stimulating factor known to mobilize stem cells from bone marrow into circulation were added to a series of experiments, which increased engraftment rates. Whether bone-marrow-derived cells or mesenchymal stem cells differentiate into olfactory cells remains controversial, necessitating the evaluation of regeneration medicine using such cells in further olfactory disorder studies.

KW - Bone marrow

KW - Olfactory

KW - Regeneration medicine

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=79957474331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79957474331&partnerID=8YFLogxK

U2 - 10.5631/jibirin.104.309

DO - 10.5631/jibirin.104.309

M3 - Article

VL - 104

SP - 309

EP - 315

JO - Practica Otologica

JF - Practica Otologica

SN - 0032-6313

IS - 5

ER -