Abstract
A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T was Gramstain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 °C. It could tolerate 0 to 2%(w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30T was most closely related to Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the mxaF, xoxF and cpn60 genes of SM30T showed its distinct phylogenetic position with respect to species with validly published names. Polymerase chain reaction (PCR) amplification of the nifH and growth on nitrogen-free medium indicated that strain SM30T is a diazotroph. The major cellular fatty acids were summed feature 8 (containing 18: 1ω7c and 18: 1ω6c) and cyclo 19: 0ω8c. The major quinone was ubiquinone 10. The DNA G+C content was 74.6 mol%. Based on the genotypic and phenotypic characteristics, strain SM30T represents a novel genus and species, for which the name Oharaeibacter diazotrophicus gen. nov., sp. nov. is proposed with the type strain SM30T (=NBRC 111955T =DSM 102969T).
| Original language | English |
|---|---|
| Article number | 001660 |
| Pages (from-to) | 576-582 |
| Number of pages | 7 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 67 |
| Issue number | 3 |
| DOIs |
|
| Publication status | Published - Apr 1 2017 |
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Keywords
- Lanthanum
- Methanol dehydrogenase
- Oharaeibacter diazotrophicus gen. nov., sp. nov
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Oharaeibacter diazotrophicus gen. nov., sp. nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere. / Lv, Haoxin; Masuda, Sachiko; Fujitani, Yoshiko; Sahin, Nurettin; Tani, Akio.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 3, 001660, 01.04.2017, p. 576-582.Research output: Contribution to journal › Comment/debate
}
TY - JOUR
T1 - Oharaeibacter diazotrophicus gen. nov., sp. nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere
AU - Lv, Haoxin
AU - Masuda, Sachiko
AU - Fujitani, Yoshiko
AU - Sahin, Nurettin
AU - Tani, Akio
PY - 2017/4/1
Y1 - 2017/4/1
N2 - A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T was Gramstain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 °C. It could tolerate 0 to 2%(w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30T was most closely related to Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the mxaF, xoxF and cpn60 genes of SM30T showed its distinct phylogenetic position with respect to species with validly published names. Polymerase chain reaction (PCR) amplification of the nifH and growth on nitrogen-free medium indicated that strain SM30T is a diazotroph. The major cellular fatty acids were summed feature 8 (containing 18: 1ω7c and 18: 1ω6c) and cyclo 19: 0ω8c. The major quinone was ubiquinone 10. The DNA G+C content was 74.6 mol%. Based on the genotypic and phenotypic characteristics, strain SM30T represents a novel genus and species, for which the name Oharaeibacter diazotrophicus gen. nov., sp. nov. is proposed with the type strain SM30T (=NBRC 111955T =DSM 102969T).
AB - A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T was Gramstain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 °C. It could tolerate 0 to 2%(w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30T was most closely related to Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the mxaF, xoxF and cpn60 genes of SM30T showed its distinct phylogenetic position with respect to species with validly published names. Polymerase chain reaction (PCR) amplification of the nifH and growth on nitrogen-free medium indicated that strain SM30T is a diazotroph. The major cellular fatty acids were summed feature 8 (containing 18: 1ω7c and 18: 1ω6c) and cyclo 19: 0ω8c. The major quinone was ubiquinone 10. The DNA G+C content was 74.6 mol%. Based on the genotypic and phenotypic characteristics, strain SM30T represents a novel genus and species, for which the name Oharaeibacter diazotrophicus gen. nov., sp. nov. is proposed with the type strain SM30T (=NBRC 111955T =DSM 102969T).
KW - Lanthanum
KW - Methanol dehydrogenase
KW - Oharaeibacter diazotrophicus gen. nov., sp. nov
UR - http://www.scopus.com/inward/record.url?scp=85016963341&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016963341&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001660
DO - 10.1099/ijsem.0.001660
M3 - Comment/debate
VL - 67
SP - 576
EP - 582
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 3
M1 - 001660
ER -