Odontoblast differentiation is regulated by an interplay between primary cilia and the canonical Wnt pathway

Kazumi Kawata, Keishi Narita, Ayako Washio, Chiaki Kitamura, Tatsuji Nishihara, Satoshi Kubota, Sen Takeda

Research output: Contribution to journalArticlepeer-review

Abstract

Primary cilium is a protruding cellular organelle that has various physiological functions, especially in sensory reception. While an avalanche of reports on primary cilia have been published, the function of primary cilia in dental cells remains to be investigated. In this study, we focused on the function of primary cilia in dentin-producing odontoblasts. Odontoblasts, like most other cell types, possess primary cilia, which disappear upon the knockdown of intraflagellar transport-88. In cilia-depleted cells, the expression of dentin sialoprotein, an odontoblastic marker, was elevated, while the deposition of minerals was slowed. This was recapitulated by the activation of canonical Wnt pathway, also decreased the ratio of ciliated cells. In dental pulp cells, as they differentiated into odontoblasts, the ratio of ciliated cells was increased, whereas the canonical Wnt signaling activity was repressed. Our results collectively underscore the roles of primary cilia in regulating odontoblastic differentiation through canonical Wnt signaling. This study implies the existence of a feedback loop between primary cilia and the canonical Wnt pathway.

Original languageEnglish
Article number116001
JournalBone
Volume150
DOIs
Publication statusPublished - Sep 2021
Externally publishedYes

Keywords

  • Canonical Wnt/β-catenin signaling pathway
  • IFT88
  • Odontoblast
  • Odontoblast differentiation
  • Primary cilia

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Histology
  • Physiology

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