Odd-skipped related 2 splicing variants show opposite transcriptional activity

Shinji Kawai, Takahiro Kato, Hiroaki Inaba, Nobuo Okahashi, Atsuo Amano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Odd-skipped related 2 (Osr2) is expressed in the domain of epithelial-mesenchymal interactions during tooth and kidney development. In this study, we report that alternative splicing of exon 4 resulted in two transcripts, Osr2A and Osr2B. These variants utilized a different splice acceptor, and Osr2B exhibited a frameshift followed by an early stop codon. Osr2A mRNA produced a protein that was 36 amino acids longer than Osr2B at the C-terminus and had five zinc-finger domains, whereas Osr2B had three zinc-finger motives. The 2 Osr2 variants were found in kidney, skeletal muscle, and testis, and Osr2B was predominantly transcribed in each. Flag-tagged variants showed the same localization in cell nuclei, however, Gal4 fusion proteins showed opposite transcriptional activities. Further, Flag-tagged variants also showed opposite transcriptional activities, though they were in contrast to the findings for the Gal4-fused variants. Our results indicate that Osr2 bifurcates its function by alternative splicing.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume328
Issue number1
DOIs
Publication statusPublished - Mar 4 2005
Externally publishedYes

Keywords

  • Alternative splicing
  • Gene expression
  • Gene structure
  • Odd-skipped related 2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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