Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen

Yoshinobu Kimura, Maiko Kamamoto, Megumi Maeda, Mitsuhiro Okano, Minehiko Yokoyama, Kosuke Kino

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1-3(Fucα1-4) GlcNAcβ1-) occurs in the N-glycans of the pollen allergens.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Juniperus ashei
Juniperus
Epitopes
Pollen
Sugars
Allergens
epitopes
Polysaccharides
polysaccharides
glycosidases
Glycoside Hydrolases
mountains
pollen
allergens
sugars
High Pressure Liquid Chromatography
Electrospray ionization
Electrospray Ionization Mass Spectrometry
reversed-phase high performance liquid chromatography
Fractionation

Keywords

  • Juniperus ashei
  • Lewis a epitope
  • Mountain cedar pollen
  • N-glycan
  • Pollen allergen

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen. / Kimura, Yoshinobu; Kamamoto, Maiko; Maeda, Megumi; Okano, Mitsuhiro; Yokoyama, Minehiko; Kino, Kosuke.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 1, 01.2005, p. 137-144.

Research output: Contribution to journalArticle

@article{40f39a899ef84599ab964a6f3574e0e4,
title = "Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen",
abstract = "We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1-3(Fucα1-4) GlcNAcβ1-) occurs in the N-glycans of the pollen allergens.",
keywords = "Juniperus ashei, Lewis a epitope, Mountain cedar pollen, N-glycan, Pollen allergen",
author = "Yoshinobu Kimura and Maiko Kamamoto and Megumi Maeda and Mitsuhiro Okano and Minehiko Yokoyama and Kosuke Kino",
year = "2005",
month = "1",
doi = "10.1271/bbb.69.137",
language = "English",
volume = "69",
pages = "137--144",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "1",

}

TY - JOUR

T1 - Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen

AU - Kimura, Yoshinobu

AU - Kamamoto, Maiko

AU - Maeda, Megumi

AU - Okano, Mitsuhiro

AU - Yokoyama, Minehiko

AU - Kino, Kosuke

PY - 2005/1

Y1 - 2005/1

N2 - We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1-3(Fucα1-4) GlcNAcβ1-) occurs in the N-glycans of the pollen allergens.

AB - We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1-3(Fucα1-4) GlcNAcβ1-) occurs in the N-glycans of the pollen allergens.

KW - Juniperus ashei

KW - Lewis a epitope

KW - Mountain cedar pollen

KW - N-glycan

KW - Pollen allergen

UR - http://www.scopus.com/inward/record.url?scp=15244359424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15244359424&partnerID=8YFLogxK

U2 - 10.1271/bbb.69.137

DO - 10.1271/bbb.69.137

M3 - Article

C2 - 15665478

AN - SCOPUS:15244359424

VL - 69

SP - 137

EP - 144

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 1

ER -