Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen

Yoshinobu Kimura, Maiko Kamamoto, Megumi Maeda, Mitsuhiro Okano, Minehiko Yokoyama, Kosuke Kino

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1-3(Fucα1-4) GlcNAcβ1-) occurs in the N-glycans of the pollen allergens.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number1
DOIs
Publication statusPublished - Jan 2005

Keywords

  • Juniperus ashei
  • Lewis a epitope
  • Mountain cedar pollen
  • N-glycan
  • Pollen allergen

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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