TY - JOUR
T1 - Occurrence of Free N-Glycans in Pea (Pisum sativum, L) Seedlings†
AU - Kimura, Yoshinobu
AU - Takagi, Shigeaki
AU - Shiraishi, Tomonori
N1 - Funding Information:
t This work was supported in part by grants from the Ministry of Education, Science, and Culture of Japan (No. 07660442, 1995-1996). tt To whom all correspondences should be addressed. Abbreviations: RP-HPLC, reverse-phase HPLC: SF-HPLC, size-fractionation HPLC; IS-MS, ion-spray mass spectrometry; MS/MS, tandem mass; PA-, pyridylamino: endo-PS, endo-{J-N-acetylglucosuminidase from sativum seed; PNGase, peptide. N-glycanase.
PY - 1997/1
Y1 - 1997/1
N2 - Free N-glycans have been found in pea seedlings. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con Α-Sepharose affinity chromatography, and size fractionation HPLC. These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ion- spray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-β-N-acetylgIucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228–232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841–1850 (1996)] during the germination of pea seeds.
AB - Free N-glycans have been found in pea seedlings. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con Α-Sepharose affinity chromatography, and size fractionation HPLC. These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ion- spray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-β-N-acetylgIucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228–232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841–1850 (1996)] during the germination of pea seeds.
KW - Endo-β-N-acetylglucosaminidase
KW - Free N-glycans
KW - Pea seedling
KW - Peptide: N-glycanase; ion-spray mass spectrometry
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U2 - 10.1271/bbb.61.924
DO - 10.1271/bbb.61.924
M3 - Article
AN - SCOPUS:0030759841
VL - 61
SP - 924
EP - 926
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 5
ER -