Occurrence of Free N-Glycans in Pea (Pisum sativum, L) Seedlings

Yoshinobu Kimura, Shigeaki Takagi, Tomonori Shiraishi

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Free N-glycans have been found in pea seedlings. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con Α-Sepharose affinity chromatography, and size fractionation HPLC. These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ion- spray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-β-N-acetylgIucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228–232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841–1850 (1996)] during the germination of pea seeds.

Original languageEnglish
Pages (from-to)924-926
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number5
DOIs
Publication statusPublished - Jan 1997
Externally publishedYes

Keywords

  • Endo-β-N-acetylglucosaminidase
  • Free N-glycans
  • Pea seedling
  • Peptide: N-glycanase; ion-spray mass spectrometry

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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