Occurrence and characteristics of class 1 and 2 integrons in clinical bacterial isolates from patients in South China

He Yan, Lin Li, Minhua Zong, Muhammad Jahangir Alam, Sumio Shinoda, Lei Shi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent studies have shown that integrons play a major role in spreading antibiotic resistance genes in clinical settings, as antibiotic resistance genes are frequently found located at gene cassettes. Polymerase chain reaction (PCR) analyses were carried out for the detection of the integrase genes of the three classes of integrons, and their gene cassettes were characterized in 118 clinical strains, including both gram-positive and gram-negative bacteria, isolated from a hospital in Guangzhou, China during 2004. Class 1 and 2 integrons were detected in 76.3% (90/118) and 0.8% (1/118) of the tested clinical isolates, respectively. Moreover four isolates were positive for both the integrons and no class 3 was detected. Some of them were first reported, such as Enterococcus faecalis, Enterococcus faecium, Alcaligenes sp., and Flavobacterium sp. Seven different arrays of gene cassettes of class 1 integron were found, and a high prevalence of dfrA12-orfF-aadA2 genes was observed. In addition, we identified a new noneopen reading frame (ORF) cassette in a class 1 integron positive clinical coagulase-negative Staphylococcus strain GH69. The none-ORF cassette, a 700 bp sequence containing a 441 bp ORF and a 59-base element, is first characterized in this study. All class 2 integrons carried an array of gene cassettes dfrA1-sat2-aadA1. Moreover bacteria contain more than one integrons were also found in this study. Resistance to trimethoprim-sulfamethoxazole was frequently found to be encoded within integrons. Therefore, it is important that guidelines for the prudent use of antimicrobial agents are adopted and surveillance programs are established.

Original languageEnglish
Pages (from-to)442-450
Number of pages9
JournalJournal of Health Science
Volume56
Issue number4
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Integrons
China
Genes
Open Reading Frames
Microbial Drug Resistance
Bacteria
Anti-Bacterial Agents
Integrases
Alcaligenes
Flavobacterium
Coagulase
Polymerase chain reaction
Enterococcus faecium
Reading Frames
Sulfamethoxazole Drug Combination Trimethoprim
Anti-Infective Agents
Enterococcus faecalis
Gram-Negative Bacteria
Staphylococcus
Guidelines

Keywords

  • Antibiotic resistance
  • Clinical isolate
  • Gene cassette
  • Integrons

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Occurrence and characteristics of class 1 and 2 integrons in clinical bacterial isolates from patients in South China. / Yan, He; Li, Lin; Zong, Minhua; Alam, Muhammad Jahangir; Shinoda, Sumio; Shi, Lei.

In: Journal of Health Science, Vol. 56, No. 4, 2010, p. 442-450.

Research output: Contribution to journalArticle

Yan, He ; Li, Lin ; Zong, Minhua ; Alam, Muhammad Jahangir ; Shinoda, Sumio ; Shi, Lei. / Occurrence and characteristics of class 1 and 2 integrons in clinical bacterial isolates from patients in South China. In: Journal of Health Science. 2010 ; Vol. 56, No. 4. pp. 442-450.
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