OBP-401-GFP telomerase-dependent adenovirus illuminates and kills high-metastatic more effectively than low-metastatic triple-negative breast cancer in vitro

Shuuya Yano, K. Takehara, Hiroyuki Kishimoto, Hiroshi Tazawa, Y. Urata, Shunsuke Kagawa, M. Bouvet, Toshiyoshi Fujiwara, R. M. Hoffman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously described the development of a highly-invasive, triple-negative breast cancer (TNBC) variant using serial orthotopic implantation of MDA-MB-231 human breast cancer in nude mice. The isolated variant is highly invasive in the mammary gland and metastasized to lymph nodes in 10 of 12 mice compared with 2 of 12 of the parental cell line. OBP-401 is a telomerase-dependent cancer-specific, green fluorescent protein (GFP)-expressing adenovirus. OBP-401 was used to infect parental MDA-MB-231P cells and high-metastatic MDA-MB-231H and MDA-MB-231HLN isolated from a lymph node metastasis and MDA-MB-231HLM isolated from a lung metastasis. Time-course imaging showed that OBP-401 labeled MDA-MB-231HP, MDA-MB-231HLN, and MDA-MB-231HLM cells more brightly than MDA-MB-231 parental cells. OBP-401 killed MDA-MB-231H, MDA-MB-231HLN, and MDA-MB-231HLM cells more efficiently than MDA-MB-231P parental cells. These results indicate that OBP-401 could infect, label and then kill high-metastatic MDA-MB-231 more efficiently than low-metastatic MDA-MB-231.

Original languageEnglish
Pages (from-to)45-47
Number of pages3
JournalCancer Gene Therapy
Volume24
Issue number2
DOIs
Publication statusPublished - Feb 1 2017

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Triple Negative Breast Neoplasms
Telomerase
Green Fluorescent Proteins
Adenoviridae
Lymph Nodes
Neoplasm Metastasis
Human Mammary Glands
Nude Mice
In Vitro Techniques
Breast Neoplasms
Cell Line
Lung
Neoplasms

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

Cite this

OBP-401-GFP telomerase-dependent adenovirus illuminates and kills high-metastatic more effectively than low-metastatic triple-negative breast cancer in vitro. / Yano, Shuuya; Takehara, K.; Kishimoto, Hiroyuki; Tazawa, Hiroshi; Urata, Y.; Kagawa, Shunsuke; Bouvet, M.; Fujiwara, Toshiyoshi; Hoffman, R. M.

In: Cancer Gene Therapy, Vol. 24, No. 2, 01.02.2017, p. 45-47.

Research output: Contribution to journalArticle

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