Abstract
We previously described the development of a highly-invasive, triple-negative breast cancer (TNBC) variant using serial orthotopic implantation of MDA-MB-231 human breast cancer in nude mice. The isolated variant is highly invasive in the mammary gland and metastasized to lymph nodes in 10 of 12 mice compared with 2 of 12 of the parental cell line. OBP-401 is a telomerase-dependent cancer-specific, green fluorescent protein (GFP)-expressing adenovirus. OBP-401 was used to infect parental MDA-MB-231P cells and high-metastatic MDA-MB-231H and MDA-MB-231HLN isolated from a lymph node metastasis and MDA-MB-231HLM isolated from a lung metastasis. Time-course imaging showed that OBP-401 labeled MDA-MB-231HP, MDA-MB-231HLN, and MDA-MB-231HLM cells more brightly than MDA-MB-231 parental cells. OBP-401 killed MDA-MB-231H, MDA-MB-231HLN, and MDA-MB-231HLM cells more efficiently than MDA-MB-231P parental cells. These results indicate that OBP-401 could infect, label and then kill high-metastatic MDA-MB-231 more efficiently than low-metastatic MDA-MB-231.
| Original language | English |
|---|---|
| Pages (from-to) | 45-47 |
| Number of pages | 3 |
| Journal | Cancer Gene Therapy |
| Volume | 24 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2017 |
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ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Cancer Research
Cite this
OBP-401-GFP telomerase-dependent adenovirus illuminates and kills high-metastatic more effectively than low-metastatic triple-negative breast cancer in vitro. / Yano, Shuuya; Takehara, K.; Kishimoto, Hiroyuki; Tazawa, Hiroshi; Urata, Y.; Kagawa, Shunsuke; Bouvet, M.; Fujiwara, Toshiyoshi; Hoffman, R. M.
In: Cancer Gene Therapy, Vol. 24, No. 2, 01.02.2017, p. 45-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - OBP-401-GFP telomerase-dependent adenovirus illuminates and kills high-metastatic more effectively than low-metastatic triple-negative breast cancer in vitro
AU - Yano, Shuuya
AU - Takehara, K.
AU - Kishimoto, Hiroyuki
AU - Tazawa, Hiroshi
AU - Urata, Y.
AU - Kagawa, Shunsuke
AU - Bouvet, M.
AU - Fujiwara, Toshiyoshi
AU - Hoffman, R. M.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - We previously described the development of a highly-invasive, triple-negative breast cancer (TNBC) variant using serial orthotopic implantation of MDA-MB-231 human breast cancer in nude mice. The isolated variant is highly invasive in the mammary gland and metastasized to lymph nodes in 10 of 12 mice compared with 2 of 12 of the parental cell line. OBP-401 is a telomerase-dependent cancer-specific, green fluorescent protein (GFP)-expressing adenovirus. OBP-401 was used to infect parental MDA-MB-231P cells and high-metastatic MDA-MB-231H and MDA-MB-231HLN isolated from a lymph node metastasis and MDA-MB-231HLM isolated from a lung metastasis. Time-course imaging showed that OBP-401 labeled MDA-MB-231HP, MDA-MB-231HLN, and MDA-MB-231HLM cells more brightly than MDA-MB-231 parental cells. OBP-401 killed MDA-MB-231H, MDA-MB-231HLN, and MDA-MB-231HLM cells more efficiently than MDA-MB-231P parental cells. These results indicate that OBP-401 could infect, label and then kill high-metastatic MDA-MB-231 more efficiently than low-metastatic MDA-MB-231.
AB - We previously described the development of a highly-invasive, triple-negative breast cancer (TNBC) variant using serial orthotopic implantation of MDA-MB-231 human breast cancer in nude mice. The isolated variant is highly invasive in the mammary gland and metastasized to lymph nodes in 10 of 12 mice compared with 2 of 12 of the parental cell line. OBP-401 is a telomerase-dependent cancer-specific, green fluorescent protein (GFP)-expressing adenovirus. OBP-401 was used to infect parental MDA-MB-231P cells and high-metastatic MDA-MB-231H and MDA-MB-231HLN isolated from a lymph node metastasis and MDA-MB-231HLM isolated from a lung metastasis. Time-course imaging showed that OBP-401 labeled MDA-MB-231HP, MDA-MB-231HLN, and MDA-MB-231HLM cells more brightly than MDA-MB-231 parental cells. OBP-401 killed MDA-MB-231H, MDA-MB-231HLN, and MDA-MB-231HLM cells more efficiently than MDA-MB-231P parental cells. These results indicate that OBP-401 could infect, label and then kill high-metastatic MDA-MB-231 more efficiently than low-metastatic MDA-MB-231.
UR - http://www.scopus.com/inward/record.url?scp=85010006405&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010006405&partnerID=8YFLogxK
U2 - 10.1038/cgt.2016.67
DO - 10.1038/cgt.2016.67
M3 - Article
C2 - 28106046
AN - SCOPUS:85010006405
VL - 24
SP - 45
EP - 47
JO - Cancer Gene Therapy
JF - Cancer Gene Therapy
SN - 0929-1903
IS - 2
ER -