NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - Protein complexes during leaf senescence

Hiroshi Yamatani, Yutaka Sato, Yu Masuda, Yusuke Kato, Ryouhei Morita, Kenji Fukunaga, Yoshiaki Nagamura, Minoru Nishimura, Wataru Sakamoto, Ayumi Tanaka, Makoto Kusaba

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57 Citations (Scopus)

Abstract

Yellowing/chlorophyll breakdown is a prominent phenomenon in leaf senescence, and is associated with the degradation of chlorophyll - protein complexes. From a rice mutant population generated by ionizing radiation, we isolated nyc4-1, a stay-green mutant with a defect in chlorophyll breakdown during leaf senescence. Using gene mapping, nyc4-1 was found to be linked to two chromosomal regions. We extracted Os07g0558500 as a candidate for NYC4 via gene expression microarray analysis, and concluded from further evidence that disruption of the gene by a translocation-related event causes the nyc4 phenotype. Os07g0558500 is thought to be the ortholog of THF1 in Arabidopsis thaliana. The thf1 mutant leaves show variegation in a light intensity-dependent manner. Surprisingly, the Fv/Fm value remained high in nyc4-1 during the dark incubation, suggesting that photosystem II retained its function. Western blot analysis revealed that, in nyc4-1, the PSII core subunits D1 and D2 were significantly retained during leaf senescence in comparison with wild-type and other non-functional stay-green mutants, including sgr-2, a mutant of the key regulator of chlorophyll degradation SGR. The role of NYC4 in degradation of chlorophyll and chlorophyll - protein complexes during leaf senescence is discussed.

Original languageEnglish
Pages (from-to)652-662
Number of pages11
JournalPlant Journal
Volume74
Issue number4
DOIs
Publication statusPublished - May 2013

Keywords

  • D1 protein
  • Oryza sativa
  • chlorophyll breakdown
  • positional cloning
  • stay-green
  • translocation

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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    Yamatani, H., Sato, Y., Masuda, Y., Kato, Y., Morita, R., Fukunaga, K., Nagamura, Y., Nishimura, M., Sakamoto, W., Tanaka, A., & Kusaba, M. (2013). NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - Protein complexes during leaf senescence. Plant Journal, 74(4), 652-662. https://doi.org/10.1111/tpj.12154