NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - Protein complexes during leaf senescence

Hiroshi Yamatani, Yutaka Sato, Yu Masuda, Yusuke Kato, Ryouhei Morita, Kenji Fukunaga, Yoshiaki Nagamura, Minoru Nishimura, Wataru Sakamoto, Ayumi Tanaka, Makoto Kusaba

    Research output: Contribution to journalArticlepeer-review

    67 Citations (Scopus)

    Abstract

    Yellowing/chlorophyll breakdown is a prominent phenomenon in leaf senescence, and is associated with the degradation of chlorophyll - protein complexes. From a rice mutant population generated by ionizing radiation, we isolated nyc4-1, a stay-green mutant with a defect in chlorophyll breakdown during leaf senescence. Using gene mapping, nyc4-1 was found to be linked to two chromosomal regions. We extracted Os07g0558500 as a candidate for NYC4 via gene expression microarray analysis, and concluded from further evidence that disruption of the gene by a translocation-related event causes the nyc4 phenotype. Os07g0558500 is thought to be the ortholog of THF1 in Arabidopsis thaliana. The thf1 mutant leaves show variegation in a light intensity-dependent manner. Surprisingly, the Fv/Fm value remained high in nyc4-1 during the dark incubation, suggesting that photosystem II retained its function. Western blot analysis revealed that, in nyc4-1, the PSII core subunits D1 and D2 were significantly retained during leaf senescence in comparison with wild-type and other non-functional stay-green mutants, including sgr-2, a mutant of the key regulator of chlorophyll degradation SGR. The role of NYC4 in degradation of chlorophyll and chlorophyll - protein complexes during leaf senescence is discussed.

    Original languageEnglish
    Pages (from-to)652-662
    Number of pages11
    JournalPlant Journal
    Volume74
    Issue number4
    DOIs
    Publication statusPublished - May 2013

    Keywords

    • D1 protein
    • Oryza sativa
    • chlorophyll breakdown
    • positional cloning
    • stay-green
    • translocation

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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