NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - Protein complexes during leaf senescence

Hiroshi Yamatani, Yutaka Sato, Yu Masuda, Yusuke Kato, Ryouhei Morita, Kenji Fukunaga, Yoshiaki Nagamura, Minoru Nishimura, Wataru Sakamoto, Ayumi Tanaka, Makoto Kusaba

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Yellowing/chlorophyll breakdown is a prominent phenomenon in leaf senescence, and is associated with the degradation of chlorophyll - protein complexes. From a rice mutant population generated by ionizing radiation, we isolated nyc4-1, a stay-green mutant with a defect in chlorophyll breakdown during leaf senescence. Using gene mapping, nyc4-1 was found to be linked to two chromosomal regions. We extracted Os07g0558500 as a candidate for NYC4 via gene expression microarray analysis, and concluded from further evidence that disruption of the gene by a translocation-related event causes the nyc4 phenotype. Os07g0558500 is thought to be the ortholog of THF1 in Arabidopsis thaliana. The thf1 mutant leaves show variegation in a light intensity-dependent manner. Surprisingly, the Fv/Fm value remained high in nyc4-1 during the dark incubation, suggesting that photosystem II retained its function. Western blot analysis revealed that, in nyc4-1, the PSII core subunits D1 and D2 were significantly retained during leaf senescence in comparison with wild-type and other non-functional stay-green mutants, including sgr-2, a mutant of the key regulator of chlorophyll degradation SGR. The role of NYC4 in degradation of chlorophyll and chlorophyll - protein complexes during leaf senescence is discussed.

Original languageEnglish
Pages (from-to)652-662
Number of pages11
JournalPlant Journal
Volume74
Issue number4
DOIs
Publication statusPublished - May 2013

Fingerprint

Chlorophyll Binding Proteins
Chlorophyll
Arabidopsis
rice
mutants
degradation
chlorophyll
leaves
Photosystem II Protein Complex
Chromosome Mapping
Microarray Analysis
Ionizing Radiation
gene targeting
ionizing radiation
Western Blotting
photosystem II
chromosome mapping
light intensity
Phenotype
Gene Expression

Keywords

  • chlorophyll breakdown
  • D1 protein
  • Oryza sativa
  • positional cloning
  • stay-green
  • translocation

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - Protein complexes during leaf senescence. / Yamatani, Hiroshi; Sato, Yutaka; Masuda, Yu; Kato, Yusuke; Morita, Ryouhei; Fukunaga, Kenji; Nagamura, Yoshiaki; Nishimura, Minoru; Sakamoto, Wataru; Tanaka, Ayumi; Kusaba, Makoto.

In: Plant Journal, Vol. 74, No. 4, 05.2013, p. 652-662.

Research output: Contribution to journalArticle

Yamatani, H, Sato, Y, Masuda, Y, Kato, Y, Morita, R, Fukunaga, K, Nagamura, Y, Nishimura, M, Sakamoto, W, Tanaka, A & Kusaba, M 2013, 'NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - Protein complexes during leaf senescence', Plant Journal, vol. 74, no. 4, pp. 652-662. https://doi.org/10.1111/tpj.12154
Yamatani, Hiroshi ; Sato, Yutaka ; Masuda, Yu ; Kato, Yusuke ; Morita, Ryouhei ; Fukunaga, Kenji ; Nagamura, Yoshiaki ; Nishimura, Minoru ; Sakamoto, Wataru ; Tanaka, Ayumi ; Kusaba, Makoto. / NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - Protein complexes during leaf senescence. In: Plant Journal. 2013 ; Vol. 74, No. 4. pp. 652-662.
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