Nucleotide sequencing and transcriptional analysis of two tandem genes encoding glucosyltransferase (water-soluble-glucan synthetase) in Streptococcus cricetus HS-6

Miho Inoue, Tetsuyoshi Inoue, Atsushi Miyagi, Ichiro Tanimoto, Ryuji Shingaki, Hiroyuki Ohta, Kazuhiro Fukui

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two tandem genes encoding glucosyltransferase synthesizing water-soluble glucan (GTF-S) were cloned from the lambda gene library of Streptococcus cricetus HS-6 (serotype a) using anti-GTF-S antibody, and the nucleotide sequences were analyzed. The two genes (ORF1 and ORF2) were identified as streptococcal glucosyltransferases based on the following evidence: [1] the deduced amino acid sequences of their products have an active site for catalytic action and C-terminal repeated units for dextran binding, and [2] a homology search revealed that the ORF1 and ORF2 products are homologous to the GtfS protein (77.4%) of S. downei Mfe28 and GtfT protein (83.8%) of S. sobrinus OMZ176, respectively, which are both known to have GTF-S activity. Therefore, ORF1 and ORF2 might be designated gtfS and gtJT of S. cricetus, respectively. A Northern blotting and RNase protection assay suggested that the gtfS and gtfT of S. cricetus are transcribed as a single bicistronic mRNA as well as separate monocistronic mRNAs. Primer extension analysis indicated multiple transcriptional start points for each gene.

Original languageEnglish
Pages (from-to)755-764
Number of pages10
JournalMICROBIOLOGY and IMMUNOLOGY
Volume44
Issue number9
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Glucosyltransferase
  • Streptococcus cricetus
  • Transcription

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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