Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid, SCP1

Haruyasu Kinashi, Miyuki Shimaji-Murayama, Tadashi Hanafusa

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SCP1 is a giant linear plasmid of 350 kb coding for the methylenomycin biosynthetic genes in Streptomyces coelicolor. The unusually long terminal inverted repeats present on both ends of SCP1 were analyzed on the nucleotide sequence level. Analysis of six clones containing the terminal 0.35-kb XbaI fragment revealed a slight heterogeneity in the nucleotide sequences of the SCP1 ends. Moreover, it was indicated that this fragment contained seven palindromic inverted repeats and a GT-rich region in the 5′-end strand. The size of the terminal inverted repeats was determined to be 81 kb by the cloning and sequencing of their end-points. An insertion sequence, IS466 was shown to be present just at the end of the right terminal inverted repeat.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
Issue number2
Publication statusPublished - 1991
Externally publishedYes


ASJC Scopus subject areas

  • Molecular Biology

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