Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA

T. Nosaka, H. Siomi, Y. Adachi, M. Ishibashi, Satoshi Kubota, M. Maki, M. Hatanaka

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The posttranscriptional regulator (rex) of human T-cell leukemia virus type I is known to be located predominantly in the cell nucleolus and to induce the accumulation of gag and env viral mRNAs. The N-terminal 19 amino acids of rex-encoded protein (Rex) has been shown to be sufficient to direct hybrid proteins to the cell nucleolus. We have studied the function of the nucleolar targeting signal (NOS) of rex by using full-length proviral DNA and mutant rex expression plasmids. Partial deletions of the NOS sequence abolished the accumulation of unspliced cytoplasmic mRNA, although the gene products of rex mutants were found in the nucleoplasm. These results indicate that NOS sequence, or nucleolar localization of Rex, is essential for Rex function.

Original languageEnglish
Pages (from-to)9798-9802
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number24
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

rex Gene Products
Cell Nucleolus
Protein Sorting Signals
Messenger RNA
Human T-lymphotropic virus 1
Plasmids
Amino Acids
DNA
Proteins
Human T-lymphotropic virus 1 rex Protein

Keywords

  • mRNA export
  • nucleolus
  • rex gene function

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA. / Nosaka, T.; Siomi, H.; Adachi, Y.; Ishibashi, M.; Kubota, Satoshi; Maki, M.; Hatanaka, M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 24, 1989, p. 9798-9802.

Research output: Contribution to journalArticle

@article{6a3d74dd8c95469a8755223222874281,
title = "Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA",
abstract = "The posttranscriptional regulator (rex) of human T-cell leukemia virus type I is known to be located predominantly in the cell nucleolus and to induce the accumulation of gag and env viral mRNAs. The N-terminal 19 amino acids of rex-encoded protein (Rex) has been shown to be sufficient to direct hybrid proteins to the cell nucleolus. We have studied the function of the nucleolar targeting signal (NOS) of rex by using full-length proviral DNA and mutant rex expression plasmids. Partial deletions of the NOS sequence abolished the accumulation of unspliced cytoplasmic mRNA, although the gene products of rex mutants were found in the nucleoplasm. These results indicate that NOS sequence, or nucleolar localization of Rex, is essential for Rex function.",
keywords = "mRNA export, nucleolus, rex gene function",
author = "T. Nosaka and H. Siomi and Y. Adachi and M. Ishibashi and Satoshi Kubota and M. Maki and M. Hatanaka",
year = "1989",
doi = "10.1073/pnas.86.24.9798",
language = "English",
volume = "86",
pages = "9798--9802",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "24",

}

TY - JOUR

T1 - Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA

AU - Nosaka, T.

AU - Siomi, H.

AU - Adachi, Y.

AU - Ishibashi, M.

AU - Kubota, Satoshi

AU - Maki, M.

AU - Hatanaka, M.

PY - 1989

Y1 - 1989

N2 - The posttranscriptional regulator (rex) of human T-cell leukemia virus type I is known to be located predominantly in the cell nucleolus and to induce the accumulation of gag and env viral mRNAs. The N-terminal 19 amino acids of rex-encoded protein (Rex) has been shown to be sufficient to direct hybrid proteins to the cell nucleolus. We have studied the function of the nucleolar targeting signal (NOS) of rex by using full-length proviral DNA and mutant rex expression plasmids. Partial deletions of the NOS sequence abolished the accumulation of unspliced cytoplasmic mRNA, although the gene products of rex mutants were found in the nucleoplasm. These results indicate that NOS sequence, or nucleolar localization of Rex, is essential for Rex function.

AB - The posttranscriptional regulator (rex) of human T-cell leukemia virus type I is known to be located predominantly in the cell nucleolus and to induce the accumulation of gag and env viral mRNAs. The N-terminal 19 amino acids of rex-encoded protein (Rex) has been shown to be sufficient to direct hybrid proteins to the cell nucleolus. We have studied the function of the nucleolar targeting signal (NOS) of rex by using full-length proviral DNA and mutant rex expression plasmids. Partial deletions of the NOS sequence abolished the accumulation of unspliced cytoplasmic mRNA, although the gene products of rex mutants were found in the nucleoplasm. These results indicate that NOS sequence, or nucleolar localization of Rex, is essential for Rex function.

KW - mRNA export

KW - nucleolus

KW - rex gene function

UR - http://www.scopus.com/inward/record.url?scp=0024829510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024829510&partnerID=8YFLogxK

U2 - 10.1073/pnas.86.24.9798

DO - 10.1073/pnas.86.24.9798

M3 - Article

C2 - 2602375

AN - SCOPUS:0024829510

VL - 86

SP - 9798

EP - 9802

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 24

ER -