Nucleo-cytoplasmic shuttling of VPg encoded by Wheat yellow mosaic virus requires association with the coat protein

Liying Sun, Bian Jing, Andika Ida Bagus, Yingchun Hu, Bingjian Sun, Rong Xiang, Hideki Kondo, Jianping Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

VPg (virus protein, genome-linked) is a multifunctional protein that plays important roles in viral multiplication in the cytoplasm. However, a number of VPgs encoded by plant viruses target the nucleus and this appears to be biologically significant. These VPgs may therefore be translocated between nuclear and cytoplasmic compartments during virus infection, but such nucleo-cytoplasmic transport has not been demonstrated. We report that VPg encoded by Wheat yellow mosaic virus (WYMV, genus Bymovirus, family Potyviridae) accumulated in both the nucleus and cytoplasm of infected cells, but localized exclusively in the nucleus when expressed alone in plants. Computational analyses predicted the presence of a nuclear localization signal (NLS) and a nuclear export signal (NES) in WYMV VPg. Mutational analyses showed that both the N-terminal and the NLS domains of VPg contribute to the efficiency of nuclear targeting. In vitro and in planta assays indicated that VPg interacts with WYMV coat protein (CP) and proteinase 1 (P1) proteins. Observation of VPg fused to a fluorescent protein and subcellular fractionation experiments showed that VPg was translocated to the cytoplasm when co-expressed with CP, but not with P1. Mutations in the NES domain or treatment with leptomycin B prevented VPg translocation to the cytoplasm when co-expressed with CP. Our results suggest that association with CP facilitates the nuclear export of VPg during WYMV infection.

Original languageEnglish
Pages (from-to)2790-2802
Number of pages13
JournalJournal of General Virology
Volume94
Issue numberPART 12
DOIs
Publication statusPublished - Dec 2013

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Mosaic Viruses
Capsid Proteins
Triticum
Genome
Viruses
Proteins
Potyviridae
Cytoplasm
Nuclear Export Signals
Nuclear Localization Signals
Cell Nucleus Active Transport
Peptide Hydrolases
Cercopithecine Herpesvirus 1
Plant Viruses
Virus Diseases
Observation

ASJC Scopus subject areas

  • Virology

Cite this

Nucleo-cytoplasmic shuttling of VPg encoded by Wheat yellow mosaic virus requires association with the coat protein. / Sun, Liying; Jing, Bian; Ida Bagus, Andika; Hu, Yingchun; Sun, Bingjian; Xiang, Rong; Kondo, Hideki; Chen, Jianping.

In: Journal of General Virology, Vol. 94, No. PART 12, 12.2013, p. 2790-2802.

Research output: Contribution to journalArticle

Sun, Liying ; Jing, Bian ; Ida Bagus, Andika ; Hu, Yingchun ; Sun, Bingjian ; Xiang, Rong ; Kondo, Hideki ; Chen, Jianping. / Nucleo-cytoplasmic shuttling of VPg encoded by Wheat yellow mosaic virus requires association with the coat protein. In: Journal of General Virology. 2013 ; Vol. 94, No. PART 12. pp. 2790-2802.
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