Nuclear dynamics of topoisomerase IIβ reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain

Akihisa Onoda, Osamu Hosoya, Kuniaki Sano, Kazuko Kiyama, Hiroshi Kimura, Shinji Kawano, Ryohei Furuta, Mary Miyaji, Ken Tsutsui, Kimiko M. Tsutsui

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Abstract

DNA topoisomerase II (topo II) changes DNA topology by cleavage/re-ligation cycle(s) and thus contributes to various nuclear DNA transactions. It is largely unknown how the enzyme is controlled in a nuclear context. Several studies have suggested that its C-terminal domain (CTD), which is dispensable for basal relaxation activity, has some regulatory influence. In this work, we examined the impact of nuclear localization on regulation of activity in nuclei. Specifically, human cells were transfected with wild-type and mutant topo IIβ tagged with EGFP. Activity attenuation experiments and nuclear localization data reveal that the endogenous activity of topo IIβ is correlated with its subnuclear distribution. The enzyme shuttles between an active form in the nucleoplasm and a quiescent form in the nucleolus in a dynamic equilibrium. Mechanistically, the process involves a tethering event with RNA. Isolated RNA inhibits the catalytic activity of topo IIβ in vitro through the interaction with a specific 50-residue region of the CTD (termed the CRD). Taken together, these results suggest that both the subnuclear distribution and activity regulation of topo IIβ are mediated by the interplay between cellular RNA and the CRD.

Original languageEnglish
Pages (from-to)9005-9020
Number of pages16
JournalNucleic acids research.
Volume42
Issue number14
DOIs
Publication statusPublished - 2014

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Type II DNA Topoisomerase
RNA
Catalytic RNA
DNA Cleavage
Enzymes
Ligation
DNA

ASJC Scopus subject areas

  • Genetics

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Nuclear dynamics of topoisomerase IIβ reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain. / Onoda, Akihisa; Hosoya, Osamu; Sano, Kuniaki; Kiyama, Kazuko; Kimura, Hiroshi; Kawano, Shinji; Furuta, Ryohei; Miyaji, Mary; Tsutsui, Ken; Tsutsui, Kimiko M.

In: Nucleic acids research., Vol. 42, No. 14, 2014, p. 9005-9020.

Research output: Contribution to journalArticle

Onoda, Akihisa ; Hosoya, Osamu ; Sano, Kuniaki ; Kiyama, Kazuko ; Kimura, Hiroshi ; Kawano, Shinji ; Furuta, Ryohei ; Miyaji, Mary ; Tsutsui, Ken ; Tsutsui, Kimiko M. / Nuclear dynamics of topoisomerase IIβ reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain. In: Nucleic acids research. 2014 ; Vol. 42, No. 14. pp. 9005-9020.
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AU - Kimura, Hiroshi

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