Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice

Research output: Contribution to journalArticle

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Abstract

Paddy rice (Oryza sativa) is able to accumulate high concentrations of Mn without showing toxicity; however, the molecular mechanisms underlying Mn uptake are unknown. Here, we report that a member of the Nramp (for the Natural Resistance- Associated Macrophage Protein) family, Nramp5, is involved in Mn uptake and subsequently the accumulation of high concentrations of Mn in rice. Nramp5 was constitutively expressed in the roots and encodes a plasma membrane-localized protein. Nramp5 was polarly localized at the distal side of both exodermis and endodermis cells. Knockout of Nramp5 resulted in a significant reduction in growth and grain yield, especially when grown at low Mn concentrations. This growth reduction could be partially rescued by supplying high concentrations of Mn but not by the addition of Fe. Mineral analysis showed that the concentration of Mn and Cd in both the roots and shoots was lower in the knockout line than in wild-type rice. A short-term uptake experiment revealed that the knockout line lost the ability to take up Mn and Cd. Taken together, Nramp5 is a major transporter of Mn and Cd and is responsible for the transport of Mn and Cd from the external solution to root cells.

Original languageEnglish
Pages (from-to)2155-2167
Number of pages13
JournalPlant Cell
Volume24
Issue number5
DOIs
Publication statusPublished - May 2012

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manganese
transporters
cadmium
uptake mechanisms
rice
growth retardation
endodermis
membrane proteins
blood proteins
Oryza sativa
macrophages
plasma membrane
Growth
grain yield
cells
toxicity
minerals
Minerals
Blood Proteins
Membrane Proteins

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice. / Sasaki, Akimasa; Yamaji, Naoki; Yokosho, Kengo; Ma, Jian Feng.

In: Plant Cell, Vol. 24, No. 5, 05.2012, p. 2155-2167.

Research output: Contribution to journalArticle

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AB - Paddy rice (Oryza sativa) is able to accumulate high concentrations of Mn without showing toxicity; however, the molecular mechanisms underlying Mn uptake are unknown. Here, we report that a member of the Nramp (for the Natural Resistance- Associated Macrophage Protein) family, Nramp5, is involved in Mn uptake and subsequently the accumulation of high concentrations of Mn in rice. Nramp5 was constitutively expressed in the roots and encodes a plasma membrane-localized protein. Nramp5 was polarly localized at the distal side of both exodermis and endodermis cells. Knockout of Nramp5 resulted in a significant reduction in growth and grain yield, especially when grown at low Mn concentrations. This growth reduction could be partially rescued by supplying high concentrations of Mn but not by the addition of Fe. Mineral analysis showed that the concentration of Mn and Cd in both the roots and shoots was lower in the knockout line than in wild-type rice. A short-term uptake experiment revealed that the knockout line lost the ability to take up Mn and Cd. Taken together, Nramp5 is a major transporter of Mn and Cd and is responsible for the transport of Mn and Cd from the external solution to root cells.

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