Novel transdermal photodynamic therapy using ATX-S10·Na(II) induces apoptosis of synovial fibroblasts and ameliorates collagen antibody-induced arthritis in mice

S. Miyazawa; K. Nishida; T. Komiyama; Y. Nakae; Ken Takeda; M. Yorimitsu; A. Kitamura; T. Kunisada; A. Ohtsuka; H. Inoue

doi:10.1007/s00296-005-0052-9

Novel transdermal photodynamic therapy using ATX-S10·Na(II) induces apoptosis of synovial fibroblasts and ameliorates collagen antibody-induced arthritis in mice

S. Miyazawa, K. Nishida, T. Komiyama, Y. Nakae, Ken Takeda, M. Yorimitsu, A. Kitamura, T. Kunisada, A. Ohtsuka, H. Inoue

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13 Citations (Scopus)

Abstract

We aimed to test the effect of transdermal photodynamic therapy (PDT) on synovial proliferation in vitro and in vivo, using a novel photosensitizer, ATX-S10·Na(II). Synovial fibroblasts were obtained from patients with RA (RASF). Cell viability with or without PDT was determined by MTT assay. Cell morphology was examined by light and transmission electron microscopy. DNA fragmentation was labeled by TUNEL stain. Collagen antibody-induced arthritis (CAIA) was induced in DBA/1 mice, and the effects of transdermal PDT were evaluated by clinical and histological examination. PDT showed drug concentration-dependent and laser dose-dependent cytotoxicity on RASF. TUNEL stain and TEM study revealed the induction of apoptotic cell death of RASF. Transdermal PDT significantly reduced clinical arthritis and synovial inflammation in this model of arthritis. These results suggest that transdermal PDT using ATX-S10·Na(II) might be a novel less invasive treatment strategy for small joint arthritis and tenosynovitis.

Original language	English
Pages (from-to)	717-725
Number of pages	9
Journal	Rheumatology International
Volume	26
Issue number	8
DOIs	https://doi.org/10.1007/s00296-005-0052-9
Publication status	Published - Jun 2006

Keywords

ATX-S10·Na(II)
Apoptosis
Fibroblast
Photodynamic therapy
Rheumatoid arthritis

ASJC Scopus subject areas

Rheumatology
Immunology and Allergy
Immunology

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10.1007/s00296-005-0052-9

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Miyazawa, S., Nishida, K., Komiyama, T., Nakae, Y., Takeda, K., Yorimitsu, M., Kitamura, A., Kunisada, T., Ohtsuka, A., & Inoue, H. (2006). Novel transdermal photodynamic therapy using ATX-S10·Na(II) induces apoptosis of synovial fibroblasts and ameliorates collagen antibody-induced arthritis in mice. Rheumatology International, 26(8), 717-725. https://doi.org/10.1007/s00296-005-0052-9

Miyazawa, S, Nishida, K, Komiyama, T, Nakae, Y, Takeda, K, Yorimitsu, M, Kitamura, A, Kunisada, T , Ohtsuka, A & Inoue, H 2006, 'Novel transdermal photodynamic therapy using ATX-S10·Na(II) induces apoptosis of synovial fibroblasts and ameliorates collagen antibody-induced arthritis in mice', Rheumatology International, vol. 26, no. 8, pp. 717-725. https://doi.org/10.1007/s00296-005-0052-9

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abstract = "We aimed to test the effect of transdermal photodynamic therapy (PDT) on synovial proliferation in vitro and in vivo, using a novel photosensitizer, ATX-S10·Na(II). Synovial fibroblasts were obtained from patients with RA (RASF). Cell viability with or without PDT was determined by MTT assay. Cell morphology was examined by light and transmission electron microscopy. DNA fragmentation was labeled by TUNEL stain. Collagen antibody-induced arthritis (CAIA) was induced in DBA/1 mice, and the effects of transdermal PDT were evaluated by clinical and histological examination. PDT showed drug concentration-dependent and laser dose-dependent cytotoxicity on RASF. TUNEL stain and TEM study revealed the induction of apoptotic cell death of RASF. Transdermal PDT significantly reduced clinical arthritis and synovial inflammation in this model of arthritis. These results suggest that transdermal PDT using ATX-S10·Na(II) might be a novel less invasive treatment strategy for small joint arthritis and tenosynovitis.",

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author = "S. Miyazawa and K. Nishida and T. Komiyama and Y. Nakae and Ken Takeda and M. Yorimitsu and A. Kitamura and T. Kunisada and A. Ohtsuka and H. Inoue",

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AU - Nakae, Y.

AU - Takeda, Ken

AU - Yorimitsu, M.

AU - Kitamura, A.

AU - Kunisada, T.

AU - Ohtsuka, A.

AU - Inoue, H.

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N2 - We aimed to test the effect of transdermal photodynamic therapy (PDT) on synovial proliferation in vitro and in vivo, using a novel photosensitizer, ATX-S10·Na(II). Synovial fibroblasts were obtained from patients with RA (RASF). Cell viability with or without PDT was determined by MTT assay. Cell morphology was examined by light and transmission electron microscopy. DNA fragmentation was labeled by TUNEL stain. Collagen antibody-induced arthritis (CAIA) was induced in DBA/1 mice, and the effects of transdermal PDT were evaluated by clinical and histological examination. PDT showed drug concentration-dependent and laser dose-dependent cytotoxicity on RASF. TUNEL stain and TEM study revealed the induction of apoptotic cell death of RASF. Transdermal PDT significantly reduced clinical arthritis and synovial inflammation in this model of arthritis. These results suggest that transdermal PDT using ATX-S10·Na(II) might be a novel less invasive treatment strategy for small joint arthritis and tenosynovitis.

AB - We aimed to test the effect of transdermal photodynamic therapy (PDT) on synovial proliferation in vitro and in vivo, using a novel photosensitizer, ATX-S10·Na(II). Synovial fibroblasts were obtained from patients with RA (RASF). Cell viability with or without PDT was determined by MTT assay. Cell morphology was examined by light and transmission electron microscopy. DNA fragmentation was labeled by TUNEL stain. Collagen antibody-induced arthritis (CAIA) was induced in DBA/1 mice, and the effects of transdermal PDT were evaluated by clinical and histological examination. PDT showed drug concentration-dependent and laser dose-dependent cytotoxicity on RASF. TUNEL stain and TEM study revealed the induction of apoptotic cell death of RASF. Transdermal PDT significantly reduced clinical arthritis and synovial inflammation in this model of arthritis. These results suggest that transdermal PDT using ATX-S10·Na(II) might be a novel less invasive treatment strategy for small joint arthritis and tenosynovitis.

KW - ATX-S10·Na(II)

KW - Apoptosis

KW - Fibroblast

KW - Photodynamic therapy

KW - Rheumatoid arthritis

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Novel transdermal photodynamic therapy using ATX-S10·Na(II) induces apoptosis of synovial fibroblasts and ameliorates collagen antibody-induced arthritis in mice

Abstract

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