Novel therapeutic transplantation of induced neural stem cells for stroke

Toru Yamashita, Wentao Liu, Yoshiaki Matsumura, Ryosuke Miyagi, Yun Zhai, Momoko Kusaki, Nozomi Hishikawa, Yasuyuki Ohta, Sung Min Kim, Tae Hwan Kwak, Dong Wook Han, Koji Abe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Somatic cells can be directly converted into induced neural stem cells (iNSCs) by defined transcription factors. However, the therapeutic effect of undifferentiated iNSCs on ischemic stroke has not been demonstrated. In this study, we used a mouse model of transient middle cerebral artery occlusion (tMCAO). iNSCs (5 ´ 105) were injected directly into the ipsilateral striatum and cortex 24 h after tMCAO. Histological analysis was performed at 7 days, 28 days, and 8 months after tMCAO. We found that iNSC transplantation successfully improved the survival rate of stroke model mice with significant functional recovery from the stroke. The fate of engrafted iNSCs was that the majority of iNSCs had differentiated into astroglial cells but not into neural cells in both the sham-operated brain and the poststroke brain without forming a tumor up to 8 months after tMCAO. Our data suggest that the directly converted iNSCs can be regarded as a candidate of safe cell resource for transplantation therapy in patients suffering from ischemic stroke.

Original languageEnglish
Pages (from-to)461-467
Number of pages7
JournalCell Transplantation
Volume26
Issue number3
DOIs
Publication statusPublished - 2017

Fingerprint

Neural Stem Cells
Stem cells
Transplantation
Stroke
Middle Cerebral Artery Infarction
Therapeutics
Brain
Transcription factors
Cell Transplantation
Stem Cell Transplantation
Therapeutic Uses
Tumors
Transcription Factors
Survival Rate
Recovery
Neoplasms

Keywords

  • Cell transplantation
  • Cerebral ischemia
  • Induced neural stem cells (iNSCs)
  • Inflammatory response

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Novel therapeutic transplantation of induced neural stem cells for stroke. / Yamashita, Toru; Liu, Wentao; Matsumura, Yoshiaki; Miyagi, Ryosuke; Zhai, Yun; Kusaki, Momoko; Hishikawa, Nozomi; Ohta, Yasuyuki; Kim, Sung Min; Kwak, Tae Hwan; Han, Dong Wook; Abe, Koji.

In: Cell Transplantation, Vol. 26, No. 3, 2017, p. 461-467.

Research output: Contribution to journalArticle

Yamashita, T, Liu, W, Matsumura, Y, Miyagi, R, Zhai, Y, Kusaki, M, Hishikawa, N, Ohta, Y, Kim, SM, Kwak, TH, Han, DW & Abe, K 2017, 'Novel therapeutic transplantation of induced neural stem cells for stroke', Cell Transplantation, vol. 26, no. 3, pp. 461-467. https://doi.org/10.3727/096368916X692988
Yamashita, Toru ; Liu, Wentao ; Matsumura, Yoshiaki ; Miyagi, Ryosuke ; Zhai, Yun ; Kusaki, Momoko ; Hishikawa, Nozomi ; Ohta, Yasuyuki ; Kim, Sung Min ; Kwak, Tae Hwan ; Han, Dong Wook ; Abe, Koji. / Novel therapeutic transplantation of induced neural stem cells for stroke. In: Cell Transplantation. 2017 ; Vol. 26, No. 3. pp. 461-467.
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